Kazuhiro Hashimoto

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Most nemaline myopathy patients have mutations in the nebulin (NEB) or skeletal muscle alpha-actin (ACTA1) genes. Here we report for the first time three patients with severe nemaline myopathy and mutations of the ACTA1 stop codon: TAG>TAT (tyrosine), TAG>CAG (glutamine) and TAG>TGG (tryptophan). All three mutations will cause inclusion of an additional 47(More)
Objective. Periapical periodontitis is an infectious and inflammatory disease of the periapical tissues caused by oral bacteria invading the root canal. In the present study, profiling of the microbiota in infected root canals was performed using anaerobic culture and molecular biological techniques for bacterial identification. Methods. Informed consent(More)
This study aimed to profile the microflora in infected root canals before and after root canal treatment using culture-independent methods. Six infected root canals in single-rooted teeth with periapical lesions from five subjects were included. Quantification of total bacteria was performed by real-time PCR with primers targeting 16S rRNA genes. PCR(More)
Design methodologies and CAD for " Emotion Engine " LSI are presented with emphasis on practical aspects of verification and timing closure. A combination of simulation, emulation and formal verification ensured the functional first silicon for system evaluation. In order to control wire delay in early design stage, floor-plan based synthesis and wire load(More)
We herein report a case of a 14-month-old infant who revealed a progressive hearing loss by repeated auditory evoked brainstem responses (ABR) during his 1 year stay in the neonatal intensive care unit (NICU). He was born prematurely with asphyxia, hyperbilirubinemia and respiratory distress. During his 1 year stay in the NICU he was under constant(More)
Coronary arteriovenous fistulas (CAF) and coronary artery aneurysms (CAA) are uncommon, but their detection has recently increased. In this case we succeeded in identifying the residual shunt flow by using an intraoperative fluorescence imaging technique with indocyanine green after surgical treatment. The photodynamic eye imaging system is based on(More)
Mutations in the epidermal growth factor receptor (EGFR) gene are associated with a favorable clinical response to the EGFR tyrosine kinase inhibitors gefitinib and erlotinib in non-small cell lung cancer (NSCLC). We present here, a new method for the rapid detection of the two most common EGFR mutations (delE746-A750 and L858R) from clinical samples. The(More)
Objective. The bacterial examination has been performed during the course of the root canal treatment. In the present pilot study, the new developed method, using fluorescence reagents and a membrane filter, was applied to the detection and quantification of bacteria in infected root canals, in order to evaluate the outcomes of the treatment. Methods. Six(More)
Processors designed for computer entertainment must perform 3D graphics calculations, especially geometry and perspective transformations. In the PlayStationR2, we introduced the new idea of synthesizing emotion called Emotion Synthesis and devised a new processor architecture to support its graphics demands. The architecture is embodied in the(More)
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