Kazuhiro Fukumori

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A temperature-responsive cross-linked polymer gel was covalently grafted onto glass coverslips by electron beam irradiation. The grafted thickness and amount of polymer as well as the surface wettability increased with the initial monomer concentration. When the monomer concentration was 5 wt.%, the grafted polymer density was 0.84microgcm(-2), and cells(More)
Ultra thin poly(N-isopropylacrylamide) (PIPAAm) modified glass coverslips (PIAPAm-CS) using electron beam irradiation exhibited a clear relationship between the polymer thickness and thermal cell adhesion/detachment behavior. The polymer thickness dependency and the characteristic of ultra thin PIPAAm layer, has been illustrated in terms of the molecular(More)
Cell-sheet-engineering based regenerative medicine is successfully applied to clinical studies, though cell sheets contain uniformly distributed cells. For the further application to complex tissues/organs, cell sheets with a multi-cellular pattern were highly demanded. Micro-contact printing is a quite useful technique for patterning proteins contained in(More)
Micro contact printing is a quite useful technique for patterning protein such as cellular adhesion molecular. Especially, the method is popular to fabricate substrates for cell patterning in tissue engineering. However, a manual printing is difficult, because the stamp made from polydimethylsiloxane (PDMS) is easily deformed, and a printed pattern is also(More)
Various thermo-responsive polymeric surfaces were evaluated in terms of cell adhesion/detachment and surface analysis. Three kinds of thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) surfaces were prepared by an electron beam irradiation (PIPAAm-EB), a reversible addition fragmentation polymerization (PIPAAm-RAFT), and a redox polymerization(More)
This study proposed a method for the fine alignment of polydimethylsiloxane (PDMS) stamp used in multiple microcontact printing. The procedure of alignment method was organized into three steps; (1) the fabrication of PDMS stamp with an alignment marker, (2) the position detection of PDMS stamp with a noncontact laser displacement sensor on an automated(More)
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