Learn More
To study whether the renin-angiotensin system is related to hyperuricemia in hypertension, the serum concentration of uric acid was determined in 96 patients with various types of hypertension and various degrees of plasma renin activity (PRA). In malignant hypertension, both PRA and the serum uric acid level were higher than in essential hypertension; but(More)
Heat shock preconditioning (HSPC) is a promising strategy for providing ischemic tolerance. The objective of this study is to investigate the effectiveness of HSPC in preventing oxidative damage of cellular proteins and DNA during ischemia-reperfusion of the liver. Male Wistar rats were divided into a heat shock group (group HS) and control (group C).(More)
Reduced tolerance of steatotic livers to ischemic injury is considered to correlate with impaired microcirculation. The aim of this study was to investigate the impact of heat-shock preconditioning (HSPC) on microcirculatory failure after ischemia/reperfusion (I/R) in steatotic livers by means of intra-vital fluorescence microscopy. Obese Zucker rats were(More)
BACKGROUND The routine transplantation of steatotic livers could potentially mitigate the donor shortage, but so far is associated with a high rate of graft dysfunction. Steatosis and brain death have been perceived as independent risk factors, but they may synergistically target the hepatic microcirculation. This study compares the effects of brain death(More)
1 Cat adrenal glands were perfused with Ca-deficient medium and secretion of catecholamines (CA) was induced by perfusion with Na-free medium in which NaCl was replaced by an osmotically equivalent amount of sucrose. 2 Release of CA and dopamine-beta-hydroxylase (DBH), but not that of phenylethanolamine-N-methyltransferase, was concomitantly found in the(More)
ATP increased the cytosolic Ca(2+) concentration ([Ca](i)) in nucleus accumbens neurons acutely dissociated from rat brain. The ATP response was dependent on external Ca(2+) and Na(+), and was blocked by voltage-dependent Ca(2+) channel blockers. The results suggest that the ATP-induced depolarization increases Ca(2+) influx resulting in the increase in(More)
Using a confocal laser-scanning microscope, we measured the changes in the cytosolic Ca(2+) concentration ([Ca]i) of chromaffin cells on adrenal slice preparations of mouse. The spontaneous fluctuations of [Ca]i were often observed in situ, as reported in isolated rat and bovine cells. Intriguingly, the spontaneous [Ca]i changes in one cell were often(More)
The rabbit adrenal gland perfused throughout with a Ca-deficient, Mg-containing medium becomes responsive to a challenge with Ca (2 mM) re-introduction after treatment with cyanide, 2-deoxy-glucose plus glucose deprivation or monoiodoacetate. The results suggest that the partial metabolic inhibition leads to the reduced activity of Na pumping, thereby(More)
To gain some understanding of the characteristics of intracellular Ca2+ stores of cat adrenal chromaffin cells, we investigated the effects of ryanodine, a blocker of Ca(2+)-induced Ca2+ release channels in muscle, on both cytosolic Ca2+ concentration and catecholamine secretion induced by caffeine or methacholine. The results suggest that Ca2+ stores(More)
To understand the mechanisms underlying the Cd2+- and Co2+-induced intracellular Ca2+ mobilization, we measured the levels of inositol phosphates using bovine chromaffin cells. Studies using HPLC indicated that Cd2+, Co2+ and methacholine significantly increased the generation of 1,4,5-IP3. The results suggest that Cd2+ and Co2+ mobilize Ca2+ from(More)