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Establishment and Characterization of Cultured Epithelial Cells Lacking Expression of ZO-1*
In well polarized epithelial cells, closely related ZO-1 and ZO-2 are thought to function as scaffold proteins at tight junctions (TJs). In epithelial cells at the initial phase of polarization,Expand
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SGIP1α Is an Endocytic Protein That Directly Interacts with Phospholipids and Eps15*
SGIP1 has been shown to be an endophilin-interacting protein that regulates energy balance, but its function is not fully understood. Here, we identified its splicing variant of SGIP1 and named itExpand
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Normal Establishment of Epithelial Tight Junctions in Mice and Cultured Cells Lacking Expression of ZO-3, a Tight-Junction MAGUK Protein
ABSTRACT ZO-1, ZO-2, and ZO-3 are closely related MAGUK family proteins that localize at the cytoplasmic surface of tight junctions (TJs). ZO-1 and ZO-2 are expressed in both epithelia andExpand
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Characterization of the EFC/F‐BAR domain protein, FCHO2
We have previously shown that SGIP1α is an endocytic protein specifically expressed in neural tissues. SGIP1α has a lipid‐binding domain called the MP domain, which shows no significant homology toExpand
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Identification and characterization of the novel centrosomal protein centlein.
Centrosomes function as the major microtubule (MT)-organizing center. They are composed of a pair of centrioles which are surrounded by the pericentriolar material. Here, we describe the molecularExpand
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Mass spectrometric analysis of microtubule co‐sedimented proteins from rat brain
Microtubules (MTs) play crucial roles in a variety of cell functions, such as mitosis, vesicle transport and cell motility. MTs also compose specialized structures, such as centrosomes, spindles andExpand
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Lipid Polarity Is Maintained in Absence of Tight Junctions*
Background: Tight junctions (TJs) are thought to prevent lipids from diffusing freely between the apical and basolateral membrane. Results: We demonstrated that lipids from the apical and basolateralExpand
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