Kazimierz Strzałka

Learn More
This paper describes violaxanthin de-epoxidation in model lipid bilayers. Unilamellar egg yolk phosphatidylcholine (PtdCho) vesicles supplemented with monogalactosyldiacylglycerol were found to be a suitable system for studying this reaction. Such a system resembles more the native thylakoid membrane and offers better possibilities for studying kinetics and(More)
We have found that in isolated spinach thylakoids, plastoquinone-pool (PQ-pool), after its photoreduction, undergoes dark-reoxidation with the half-time of τ1/2 = 43 ± 3 s. To explain the observed rates of PQ-pool reoxidation, a nonenzymatic plastoquinol (PQH2) autoxidation under molecular oxygen and an enzymatic oxidation by the low-potential form of(More)
The molecular background of Hg (2+)-induced inhibition of protochlorophyllide (Pchlide) photoreduction was investigated in homogenates of dark-grown wheat leaves. Our earlier work showed that 15 min incubation with 10 (-2) M Hg (2+) completely inhibits the activity of NADPH : Pchlide oxidoreductase ( ). Detailed analysis of spectra recorded at 10 K(More)
Carotenoids are a group of pigments present both in the plant and animal kingdoms, which play several important physiological functions. The protection against active oxygen species, realised via the quenching of excited states of photosensitizing molecules, quenching of singlet oxygen and scavenging of free radicals, is one of the main biological functions(More)
Lipocalins are a widely distributed group of proteins whose common feature is the presence of six-or eight-stranded beta-barrel in their tertiary structure and highly conservative motifs short conserved region, (SCR) in their amino acid sequences. The presence of three SCRs is typical for kernel lipocalins, while outlier lipocalins have only one or two such(More)
Spin label EPR studies (Strzałka and Gruszecki (1994) Biochim. Biophys. Acta 1194, 138-142) revealed that beta-carotene affects structural and dynamic properties of model dipalmitoylphosphatidylcholine (DPPC) membranes (multilamellar liposomes) more than polar carotenoid lutein. NMR measurements presented in this paper demonstrate that beta-carotene exerts(More)
The light-dependent, cyclic changes of xanthophyll pigments: violaxanthin, antheraxanthin and zeaxanthin, called the xanthophyll cycle, have been known for about fifty years. This process was characterised for higher plants, several fern and moss species and in some algal groups. Two enzymes, violaxanthin de-epoxidase (VDE) and zeaxanthin epoxidase (ZE),(More)
We have shown that the isolated 33 kDa protein of photosystem II contains one calcium and one lanthanide low-affinity binding site with binding constants (K(D)) on the order of 10(-5) M. Binding of calcium or lanthanides to this site induces conformational changes in the protein that manifest in fluorescence emission spectra of the protein, circular(More)
The surface pressure-area isotherms of pure plastoquinone-9 (PQ-9), plastoquinone-3 (PQ-3), alpha-tocopherol quinone (alpha-TQ), their reduced (hydroquinone) forms and mixtures of these molecules with monogalactosyldiacylglycerol (MGDG) have been studied by a monolayer technique. The collapse pressures of all hydroquinones (QH2) were higher than those of(More)
In the present study the influence of the lipid environment on the organization of the main light-harvesting complex of photosystem II (LHCII) was investigated by 77K fluorescence spectroscopy. Measurements were carried out with a lipid-depleted and highly aggregated LHCII which was supplemented with the different thylakoid membrane lipids. The results show(More)