Katy M. Coxon

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Nerve cell death is the key event in all neurodegenerative disorders, with apoptosis and necrosis being central to both acute and chronic degenerative processes. However, until now, it has not been possible to study these dynamically and in real time. In this study, we use spectrally distinct, well-recognised fluorescent cell death markers to enable the(More)
Anuphon Laohavisit,a Jennifer C. Mortimer,a,1,2 Vadim Demidchik,a,1,3 Katy M. Coxon,a,4 Matthew A. Stancombe,a Neil Macpherson,a Colin Brownlee,b Andreas Hofmann,c Alex A.R. Webb,a Henk Miedema,d Nicholas H. Battey,e and Julia M. Daviesa,5 a Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom bMarine Biological(More)
Pantothenate (vitamin B5) is a water-soluble vitamin essential for the synthesis of CoA and ACP (acyl-carrier protein, cofactors in energy yielding reactions including carbohydrate metabolism and fatty acid synthesis. Pantothenate is synthesized de novo by plants and micro-organisms; however, animals obtain the vitamin through their diet. Utilizing our(More)
Plant cell growth and stress signaling require Ca²⁺ influx through plasma membrane transport proteins that are regulated by reactive oxygen species. In root cell growth, adaptation to salinity stress, and stomatal closure, such proteins operate downstream of the plasma membrane NADPH oxidases that produce extracellular superoxide anion, a reactive oxygen(More)
Under-representation of teenagers and young adults in clinical trials for cancer is acknowledged internationally and might account for the lower survival gains noted for this group. Little research has focused on strategies to increase participation of teenagers and young adults in clinical trials. We applied a conceptual framework for barriers to(More)
Cell death by apoptosis has been studied for many years using fluorescently labeled annexin V. Annexin V shows high affinity for the phosphatidylserine that becomes enriched in the outer leaflet of the plasma membrane during apoptosis, but not necrosis, allowing differentiation between the two types of cell death. In this chapter we detail two methods for(More)
Laohavisit, A., Shang, Z., Rubio, L., Cuin, T. A., Very, A.-A., Wang, A., Mortimer, J. C., Macpherson, N., Coxon, K. M., Battey, N. H., Brownlee, C., Park, O. K., Sentenac, H., Shabala, S., Webb, A. A. R. and Davies, J. M. (2012) Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+ and K+ -permeable conductance in root cells. The Plant(More)
Annexins are cytosolic proteins capable of reversible, Ca(2+)-dependent membrane binding or insertion. Animal annexins form and regulate Ca(2+)-permeable ion channels and may therefore participate in signaling. Zea mays (maize) annexins (ZmANN33 and ZmANN35) have recently been shown to form a Ca(2+)-permeable conductance in planar lipid bilayers and also(More)
Pantothenate (vitamin B5) is the precursor of the 4′-phosphopantetheine moiety of coenzyme A and acyl-carrier protein. It is made by plants and microorganisms de novo, but is a dietary requirement for animals. The pantothenate biosynthetic pathway is well-established in bacteria, comprising four enzymic reactions catalysed by ketopantoate(More)