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PURPOSE To characterize the intracellular signaling mechanism that underlies the contraction of trabecular meshwork (TM) tissue. METHODS The contraction of collagen mediated by bovine TM cells was evaluated by measuring changes in the diameter of collagen gels in which the cells were embedded. Changes in the organization of the actin cytoskeleton were(More)
PURPOSE Excessive wound contraction can lead to scar formation in the conjunctiva. The effects of all-trans-retinoic acid (ATRA) on the contractility of human Tenon fibroblasts (HTFs) cultured in three-dimensional (3D) collagen gels were investigated. METHODS Human Tenon fibroblasts were cultured in 3D gels of type I collagen and in the absence or(More)
The corneal epithelium serves as a barrier and contributes to the maintenance of corneal transparency and rigidity. In most instances, corneal epithelial defects caused by simple injury are resurfaced promptly. However, in individuals with certain clinical conditions, such as herpes simplex virus infection, neurotrophic keratopathy or diabetic keratopathy,(More)
The effects of algesic compounds on the distal portion of the processes of cultured dorsal root ganglion cells (C-fiber) of mouse were studied by patch-clamp whole-cell recording at the cell soma (cell body). The processes of the cell were isolated from the cell body with a separator. Bradykinin (BK, 10 microM), prostaglandin E2 (PGE2, 20 microM), and(More)
The immunocytochemical distribution of manganese superoxide dismutase (Mn-SOD) was determined in the rat hippocampus. The enzyme was localized in the mitochondria. CA1 pyramidal cells were weakly immunostained, whereas CA3 pyramidal cells were strongly reactive. These differences in the intensity of the Mn-SOD immunostaining reactions may relate to(More)
PURPOSE To characterize changes in the localizations of the basement membrane protein laminin-1 and of adhesion proteins of intercellular junctions during wound healing after epithelial ablation in the rat cornea. METHODS Epithelial ablation was performed with an excimer laser. Rats were killed immediately, 12 hours, 24 hours, 3 days, or 4 weeks after(More)
The effects of Ga-Al-As diode laser (830 nm, 16.2 mW) irradiation on the distal portion of the processes of cultured murine dorsal root ganglion (DRG) neurons associated with C-fibers were studied by patch-clamp whole-cell recording of membrane potentials at the cell body. The chemical as well as laser light stimulations were limited to the processes of the(More)
PURPOSE To investigate the expression of functional gap junctions and the effect of protein kinase C (PKC) on such junctions in confluent cultures of bovine trabecular meshwork (TM) cells. METHODS Expression of the gap junction protein connexin43 in TM cells was examined by immunofluorescence microscopy. Intercellular communication by gap junctions was(More)
BACKGROUND Treatments for open-angle glaucoma aim to prevent vision loss through lowering of intraocular pressure, but to our knowledge no placebo-controlled trials have assessed visual function preservation, and the observation periods of previous (unmasked) trials have typically been at least 5 years. We assessed vision preservation in patients given(More)
We used rabbit antisera against manganese (Mn)-superoxide dismutase for immunohistochemical studies of localization in the rat neostriatum. Immunostaining was intense in large-sized neurons and several medium-sized neurons, but it was moderate to weak in other cells. Double immunostaining with monoclonal antibody to choline acetyltransferase or somatostatin(More)