Katsutoshi Takahashi

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MOTIVATION The modeling of system dynamics of genetic networks, metabolic networks or signal transduction cascades from time-course data is formulated as a reverse-problem. Previous studies focused on the estimation of only network structures, and they were ineffective in inferring a network structure with feedback loops. We previously proposed a method to(More)
We have developed the fully-automated algorithms for processing 2-D gel electrophoretograms based on RLGS (restriction landmark genomic scanning) method; one for fully-automated spot recognition from RLGS electrophoretogram and another for fully-automated pairwise matching of the spots found on such 2-D electrophoretograms. Without any human interaction,(More)
Single particle analysis is a straightforward method for studying the structures of macromolecules that cannot be crystallized. It builds three-dimensional structures of particles by estimating the projection angles of their randomly oriented electron-microscopic images. The existing methods divide the images into clusters, build class averages for the(More)
We have developed a p owerful image processing system DNAinsight, which performs automated detection of several thousands of spots found on autoradiogram images obtained with 2-D gel elec-trophoresis of genomic DNA. Algorithms and parameters for detecting spot locations and intensities are c arefully chosen so as to enable reliable and rapid processing of(More)
We have developed the automated processing algorithms for 2-dimensional (2-D) electrophore-tograms of genomic DNA based on RLGS (Restriction Landmark Genomic Scanning) method, which scans the restriction enzyme recognition sites as the landmark and maps them onto a 2-D electrophoresis gel. Our powerful processing algorithms realize the automated spot(More)
DNA methylation occurring within the context of CpG site in the promoter element generally correlates with transcriptional silencing, delayed replication, condensed chromatin and mammalian genome imprinting (Nature Genet 24:101-2, 2000). We have been focusing attention on Restriction Landmark Genomic Scanning (RLGS) method as the powerful experimental(More)