Katrina M. Beesley

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Fragment C is a non-toxic 50 kDa fragment of tetanus toxin which is a candidate subunit vaccine against tetanus. The AT-rich Clostridium tetani DNA encoding fragment C could not be expressed in Saccharomyces cerevisiae due to the presence of several fortuitous polyadenylation sites which gave rise to truncated mRNAs. The polyadenylation sites were(More)
Epitopes defined by monoclonal antibodies (mAb) specific for the Bordetella pertussis outer membrane protein P.69 (pertactin) were mapped using a series of amino- and carboxy-terminal deletion mutants expressed in Escherichia coli. mAb were found to bind predominantly to a region of pertactin spanning a (Pro-Gln-Pro)5 repeat motif and one mAb was found to(More)
Hepatitis B core protein (HBcAg) is a potent antigen that gives both a T-cell dependent and a T-cell-independent antibody response. It has been shown that a foreign epitope can be fused to the amino terminus of HBcAg without affecting partide integrity, and that the resulting chimaeric cores retain the immunogenicity of the foreign epitope. Here we describe(More)
Acellular whooping cough vaccines are based on pertussis toxoid but their effectiveness may be increased by the addition of other Bordetella pertussis antigens. We expressed the immunogenic outer membrane protein pertactin (P69) from B. pertussis to high levels in multi-copy transformants of the industrial yeast Pichia pastoris. In high-density(More)
Yeast expression vectors are generally based on the 2 micron plasmid and are not stably inherited, so that continued selection pressure must be applied to prevent the gradual accumulation of plasmid-free cells. A number of 'autoselection' systems have been proposed and perhaps the most versatile is the stabilisation of plasmids containing the URA3 gene in(More)
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