Katrina M. Beesley

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Yeast expression vectors are generally based on the 2 micron plasmid and are not stably inherited, so that continued selection pressure must be applied to prevent the gradual accumulation of plasmid-free cells. A number of 'autoselection' systems have been proposed and perhaps the most versatile is the stabilisation of plasmids containing the URA3 gene in(More)
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