Katrin Birkholz

  • Citations Per Year
Learn More
A recombinant immunotoxin was constructed by fusing a single chain fragment variable antibody fragment, specific for the melanoma-associated chondroitin sulfate proteoglycan (MCSP), to a truncated variant of Pseudomonas exotoxin A (ETA'), carrying a C-terminal KDEL-peptide for improved retrograde intracellular transport. The resulting immunotoxin MCSP-ETA'(More)
Human T lymphocytes can be redirected with a new defined specificity by expression of a chimeric T-cell receptor (immunoreceptor) for the use in adoptive immunotherapy of cancer. Whereas standard procedures use retroviral gene transduction to constitutively express immunoreceptors in T cells, we here explored for the first time mRNA electroporation to(More)
The use of dendritic cells (DCs) in therapeutic cancer vaccination requires their loading with tumor-specific antigen(s). DEC-205, a phagocytosis receptor mediating antigen uptake, is associated with CD8(+) T-cell responses in mice. Here we fused an anti-DEC-205scFv to an HLA-DP4-restricted epitope from the tumor antigen MAGE-A3, and examined the(More)
Chimeric antigen receptor (CAR)-modified T cells emerged as effective tools in the immunotherapy of cancer but can produce severe on-target off-tissue toxicities. This risk can conceivably be overcome, at least partially, by transient transfection. The design of CARs, however, has so far not been optimized for use in non-permanent T cell modification. Here(More)
Sequencing, cloning and functional testing of T-cell-receptor (TCR) alpha- and beta-chains from T-cell clones is often required in immunotherapy and in immunological research. However, the determination of the TCR chains by a simple PCR is not possible, since, in contrast to the 3' constant domain and untranslated region (UTR), no conserved sequences are(More)
(addition to “Cancer vaccination in general”) Pierre Coulie (Université catholique de Louvain, Belgium) introduced data from vaccination trials with a MAGE-A3 peptide or with ALVAC-MAGE (a canarypox virus containing MAGE-A3 and -A1 minigenes) about the numbers of anti-vaccine T cells. Frequencies of antiMAGE-A3 CD8 T cells in blood were low (around 10¡5),(More)
The international meeting of the Collaborative Research Center ‘‘Strategies of cellular immune intervention’’ (SFB 643) was held in Erlangen, Germany, on July 17–18, 2006. The main aim of this Collaborative Research Center is to translate knowledge on cellular immunology, acquired by basic research, into new therapeutic strategies for diseases. This report(More)
  • 1