Katriina Aalto-Setala

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To determine the physiological role of apolipoprotein (apo) A-IV, knockout mice were created by gene targeting in embryonic stem cells. In apoA-IV knockout mice, plasma cholesterol and triglyceride levels were reduced 25% and 44%, respectively, compared with controls. These changes were accounted for by decreased high density (HDL) and very low density(More)
Human pluripotent stem cell-derived hepatocytes have the potential to provide in vitro model systems for drug discovery and hepatotoxicity testing. However, these cells are currently unsuitable for drug toxicity and efficacy testing because of their limited expression of genes encoding drug-metabolizing enzymes, especially cytochrome P450 (CYP) enzymes.(More)
Video-based analysis of cardiomyocytes provides a non-invasive and label-free method of analyzing their beating characteristics. Here, we aim to demonstrate that defining averaged signal templates can improve the determination of cardiomyocyte beating characteristics. Video recordings of human iPSC derived cardiomyocytes were performed Beating patterns from(More)
BACKGROUND Family history of myocardial infarction (MI) is an independent risk factor for MI. Several genetic variants are associated with increased risk of MI and family history of MI in a first-degree relative doubles MI risk. However, although family history of MI is not a simple dichotomous risk factor, the impact of specific, detailed family histories(More)
BACKGROUND The use of next-generation sequencing enables a rapid analysis of many genes associated with sudden cardiac death in diseases like Brugada Syndrome. Genetic variation is identified and associated with 30-35% of cases of Brugada Syndrome, with nearly 20-25% attributable to variants in SCN5A, meaning many cases remain undiagnosed genetically. To(More)
We present detailed quantitative measurement analyses for flow in a spinner flask with spinning rates between 20 to 45 RPM, utilizing the optical velocimetry measurement technique of Particle Image Velocimetry (PIV). A partial section of the impeller was immersed in the working fluid to reduce the shear forces induced on the cells cultured on microcarriers.(More)
BACKGROUND Numerous risk prediction algorithms based on conventional risk factors for Coronary Heart Disease (CHD) are available but provide only modest discrimination. The inclusion of genetic information may improve clinical utility. METHODS We tested the use of two gene scores (GS) in the prospective second Northwick Park Heart Study (NPHSII) of 2775(More)
BACKGROUND Several randomized controlled trials (RCTs) have evaluated the effect of intra-aortic balloon counterpulsation pump(IABP) on the mortality of acute myocardial infarction (AMI). OBJECTIVES To analyze the relevant RCT data on the effect of IABP on mortality and the occurrence of bleeding in AMI. DATA SOURCES Published RCTs on the treatment of(More)
The aim of the present study was to evaluate the effect of variation of different gene loci separately and in concert on lipid metabolism in heterozygous familial hypercholesterolemia (FH). We assayed a unique low density lipoprotein (LDL) receptor gene defect (designated as FH-Helsinki), the Xba I polymorphism of the apolipoprotein (apo) B, phenotypes of(More)
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) represent a potential valuable alternative to human embryonic stem cell-derived cardiomyocytes (hESC-CMs) for the lack of ethical issues and their patient-specificity. Recent studies showed hiPSC-CMs behave like hESC-CMs in terms of membrane currents, contractility and frequency of(More)