Katja Heusser

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Arginine (R)-based ER localization signals are sorting motifs that confer transient ER localization to unassembled subunits of multimeric membrane proteins. The COPI vesicle coat binds R-based signals but the molecular details remain unknown. Here, we use reporter membrane proteins based on the proteolipid Pmp2 fused to GFP and allele swapping of COPI(More)
Arginine (Arg)-based endoplasmic reticulum (ER)-localization signals are involved in the quality control of different heteromultimeric membrane protein complexes. ATP-sensitive potassium (KATP) channels are unique because each subunit in the heterooctamer contains an Arg-based ER-localization signal. We have dissected the inactivation events that override(More)
Recent progress in our understanding of the trafficking of potassium channels can be seen in particular when considering the Kv-type channels. To date, we have discovered that folding of the Kv1.3 T1 domain begins in the ribosomal exit tunnel, and that the cell surface expression of Kv4 channels is enhanced by the presence of two recently identified(More)
The two-pore-domain potassium channels TASK-1 (KCNK3) and TASK-3 (KCNK9) modulate the electrical activity of neurons and many other cell types. We expressed TASK-1, TASK-3 and related reporter constructs in Xenopus oocytes, mammalian cell lines and various yeast strains to study the mechanisms controlling their transport to the surface membrane and the role(More)
pL569 PMP2-GFP-LRKRSin p416 Michelsen et al., 2006 pG350 PMP2-GFP-LRKRSin p415 This study pL571 PMP2-GFP-KKXX in p416 This study pR866 PMP2-GFP-KKXX in p415 This study pL570 PMP2-GFP-AAAASin p416 Michelsen et al., 2006 pL572 PMP2-GFP-AAXX in p416 This study pM650 PMP2-YFP-LRKRSin p416 Michelsen et al., 2006 pN654 PMP2-YFP-KKXX in p416 Michelsen et al., 2006(More)
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