Katie Fraass Wlaschin

Learn More
DNA microarray based transcriptome analysis has become widely used in biomedical research; however, the lack of DNA sequence information available for Chinese hamster ovary (CHO) cells has hampered the application of microarrays for this cell line widely used for recombinant therapeutic protein production. We have constructed an expressed sequence tag (EST)(More)
Recombinant mammalian cells are used to produce numerous, high-value protein therapeutics. Generating hyper-producing cell lines is crucial for delivery of products to ailing patients. Better understanding of the complex trait of hyperproductivity can facilitate the creation of hyper-producing cell lines. Ruminating over the reported transcriptomic and(More)
DNA microarray technology has been widely utilized for species with extensive genome sequence information available. Given the limited genomic information pertaining to Chinese hamster ovary (CHO) cell line, cross-species hybridization using mouse microarrays provides a viable alternative. In this study, the utility of mouse Affymetrix microarrays for(More)
Transporters mediate the influx of nutrients and excretion of metabolites in mammalian cells, playing a key role in the regulation of metabolism. They are natural targets for cell engineering to alter metabolic characteristics. The GLUT5 fructose transporter was stably expressed in a Chinese hamster ovary cell line, allowing clones to utilize fructose in(More)
Chinese hamster ovary (CHO) cells are one of the most important cell lines in biological research, and are the most widely used host for industrial production of recombinant therapeutic proteins. Despite their extensive applications, little sequence information is available for molecular based research. To facilitate gene discovery and genetic engineering,(More)
In the past decade, we have seen a rapid expansion in mammalian cell based therapeutic proteins reaching clinical applications. This increased demand has been met with much increased productivity through intensive process development. During this time, fedbatch culture processes have emerged as the predominant mode for producing recombinant proteins. In(More)
Chinese hamster ovary (CHO) cells are a prevalent tool in biological research and are among the most widely used host cell lines for production of recombinant therapeutic proteins. While research in other organisms has been revolutionized through the development of DNA sequence-based tools, the lack of comparable genomic resources for the Chinese hamster(More)
Genomic and proteomic based global gene expression profiling has altered the landscape of biological research in the past few years. Its potential impact on cell culture bioprocessing has only begun to emanate, partly due to the lack of genomic sequence information for the most widely used industrial cells, Chinese hamster ovary (CHO) cells. Transcriptome(More)
  • 1