Kathi Westphal

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Organ failure in Plasmodium falciparum malaria is associated with neutrophil activation and endothelial damage. This study investigates whether neutrophil-induced endothelial damage involves apoptosis and whether it can be prevented by neutralization of neutrophil secretory products. Endothelial cells from human umbilical veins were coincubated with(More)
Wound healing is a complex process in which a tissue's individual cells have to be orchestrated in an efficient and robust way. We integrated multiplex protein analysis, immunohistochemical analysis, and whole-slide imaging into a novel medium-throughput platform for quantitatively capturing proliferation, differentiation, and migration in large numbers of(More)
BACKGROUND Determining the correct number of positive immune cells in immunohistological sections of colorectal cancer and other tumor entities is emerging as an important clinical predictor and therapy selector for an individual patient. This task is usually obstructed by cell conglomerates of various sizes. We here show that at least in colorectal cancer(More)
Cutaneous regeneration utilizes paracrine feedback mechanisms to fine-tune the regulation of epidermal keratinocyte proliferation and migration. However, it is unknown how fibroblast-derived hepatocyte growth factor (HGF) affects these mutually exclusive processes in distinct cell populations. We here show that HGF stimulates the expression and(More)
MOTIVATION For a mechanistic understanding of skin and its response to an induced perturbation, systems biology is gaining increasing attention. Unfortunately, quantitative and spatial expression data for skin, like for most other tissues, are almost not available. RESULTS Integrating organotypic skin cultures, whole-slide scanning and subsequent image(More)
We developed a method for the quantification of skin irritation based on the treatment of skin cultures and the subsequent automatic image analysis of fluorescently stained tissue sections being scanned with a fullslide scanner. In this method, the irritative effect is reflected in a shift of the expression pattern of selected biomarkers inside the(More)
The development of intrapulmonary myelin sheaths began in the central parts of the lung and proceeded towards the periphery. It started suddenly on the 25th post conceptional (p.c.) day. Early stages of myelination could be demonstrated in the periphery on the 27th day p.c (2) Axons not surrounded by Schwann cells could be seen in large numbers on the 24th(More)
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