Katerina Ruzickova

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BACKGROUND AIMS Human mesenchymal stromal cells (hMSC) are a promising tool for future clinical application, but their use requires rapid cell expansion in media suitable for clinical use. Therefore, we tested the influence of several culture media on colony formation, population doubling (PD) time, cell cycle and surface marker expression. METHODS hMSC(More)
BACKGROUND AIMS Olfactory ensheathing glia (OEG) and mesenchymal stromal cells (MSC) are suitable candidates for transplantation therapy of spinal cord injury (SCI). Both facilitate functional improvement after SCI by producing trophic factors and cytokines. In this study, the co-transplantation of both types of cells was studied to clarify their additive(More)
Metabolite changes in an experimental lesion in the rat cortex and in the contralateral hemisphere after the intravenous administration of mesenchymal stem cells (MSCs) were assessed by proton MR spectroscopy to verify the impact of the cell treatment on the brain tissue. Wistar rats with a photochemical cortical lesion and transplanted MSCs or sham(More)
Amyotrophic lateral sclerosis (ALS) is a progressive untreatable neurodegenerative disorder, leading to the death of the cortical and spinal motoneurons (MNs). Bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs) may represent a new approach to slowing down the progression of ALS by providing neurotrophic support to host MNs and by having an(More)
Preventive vaccination by a hsp90-expressing DNA vaccine and recombinant hsp90 protein vaccine, both derived from theCandida albicans hsp90 using BALB-c mouse model of systemic candidiasis, was performed. Hsp90 mRNA was cloned from a clinical isolate ofC. albicans, converted to cDNA and cloned into vaccination plasmid pVAX1. Two methods of DNA application(More)
The paper describes an expert system that helps in a process of a map sheet creation. The expert system is based on ontology that describes basic elements of a map sheet and geodata used for map sheet creation. The paper describes mainly how is the ontology used for building knowledge base of the expert system. The expert system is based on DROOLS software.(More)
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