Katarzyna Pawlak

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The mechanism by which the most relevant ruthenium anticancer drugs are activated in tumors to commence their tumor-inhibiting action remains one of the challenging research tasks of present-day metallomics. This contribution aims to capture and identify eventually more reactive species of one of two bis-indazole tetrachloridoruthenate(III) compounds that(More)
Mutation of the heat shock gene, htpG, causes severe defects of several cellular functions in Escherichia coli. A null htpG mutant constructed by gene replacement was impaired in the biosynthesis and secretion of several enzymes, and in biofilm formation and proteolysis. A significant decrease in the activity of β-lactamase in the ΔhtpG mutant was observed(More)
Electrospray ionization mass spectrometry (ESI-MS) techniques have been used to characterize the speciation of a Ru(III) anticancer drug, indazolium trans-[tetrachloridobis(1H-indazole) ruthenate(III)], upon its binding to transferrin and the impact of cellular reducing components on drug-transferrin adducts. Using time-of-flight ESI-MS, the polymorphism of(More)
The discovery of intracellular active forms is a crucial issue for the approval of further anticancer metal-based drugs. This challenge calls for an apt analytical methodology to scrutinize the speciation changes of a metallodrug in cancer cytosol. In the current study, we have developed an approach for portraying low-molecular-mass cytosolic species of a(More)
This perspective article highlights the potential of capillary electrophoresis (CE) in in-line monitoring of biomolecular reactions related to in vivo transformations of metal species. In such scrutinizing, the capillary is regarded as a nanolitre-volume reactor in which electrical field-driven reactants are mixed to produce a response that enables in situ(More)
The mode of action of metal-based anticancer drugs, including their accumulation in blood, transport, delivery to cancer cell, and cell processing (together with release of an active form and possibly targeting) is largely dependent on protein binding. Among analytical methods capable of providing a better understanding of metallodrug-protein interactions,(More)
Size-exclusion chromatography (SEC) and capillary zone electrophoresis (CZE) coupled with inductively coupled plasma mass spectrometry were applied to characterize low, medium, and high molecular weight cadmium complexes with glutathione and phytochelatins (PCs). The dominant stoichiometry of the complexes formed in vitro was established as 1:1 using(More)
Chiral additives in the nematic liquid crystal can alter the dynamics of point defects moving on a disclination line. They exert a constant force on defects, leading to the bimodal distribution of distances between them at long times. The evolution of the system of defects in the presence of chiral additives provides a very direct proof of the existence of(More)
An Escherichia coli hemH mutant accumulates protoporphyrin IX, causing photosensitivity of cells to visible light. Here, we have shown that intracellular free iron in hemH mutants is double that observed in hemH(+) strain. The aim of this study was to recognize the influence of this increased free iron concentration on AlkB-directed repair of alkylated DNA(More)