Katarína Mihaliková

Learn More
AIMS To increase rumen output of γ-linolenic acid (GLA), we used two cereal-derived substrates, ground barley (GB) and rye bran (RB), enriched with fungal GLA as components of feed rations. We examined their effects on rumen fermentation patterns, lipid metabolism and the ciliated protozoan population in an artificial rumen. METHODS AND RESULTS Four diets(More)
Entodiniomorphid ciliates are intestinal protists inhabiting the colons of African great apes. The participation of intestinal entodiniomorphid ciliates in ape hindgut digestion has been proposed, but little data have been available to support the hypothesis. We measured the specific activities of carboxymethyl cellulase, xylanase, inulinase, and α-amylase(More)
Galvanotaxis was shown to be an efficient method for cleaning and concentrating rumen ciliate protozoa whose harvesting (centrifugation of large volumes ofin vitro cultures followed by repeated washing of the sediment to remove plant debris) is time consuming. We suggested the use of a new galvanotaxis apparatus (a small-capacity two-way glass stopcock) to(More)
The effect of selenium supplementation on the rumen protozoan population of sheep was demonstrated. Both the total and generic counts of rumen ciliates in sheep fed a diet with basal Se content (70 µg/kg dry matter) were compared to those of animals given feed supplemented with inorganic (disodium selenite) or organic Se (selenized yeast) (310 µg/kg dry(More)
The objective of this experiment was to investigate the effects of feed supplementation with equivalent doses of selenium from sodium selenite (SS) or selenized yeast (SY) on Se deposition, selenoenzyme activity and lipid peroxidation in tissues as well as in bacterial and protozoal fractions of rumen contents in sheep. The phagocytic activity of monocytes(More)
The effects of diets (i.e., high fibre, HF and high concentrate, HC) supplemented with either fumaric acid (FUM; 8 mmol/L) or maleic acid (MAL; 8 mmol/L) and different plant oils (35.0 g/kg dry matter; sunflower, SO; rapeseed, RO; and linseed, LO) on rumen sheep fatty acids (FA) concentration and biohydrogenation (BH) in batch cultures were examined. The(More)
We examined the role of rumen ciliates, using Entodinium caudatum as a model organism, in the detoxification of soluble mercury(II) in vitro under conditions with enhanced or reduced diversity of a co-culture bacterial population as well as the effects of long-term mercury(II) stress on in vitro fermentation parameters and major mercury detoxification(More)
Stobadin (STO) is a prospective cardioprotective drug with antiarrhythmic and antihypoxic effects on the myocardium. Single iv injections of stobadin administered to rats on days 3, 6, 9 or 12 of gestation at doses of 2 and 6 mg/kg had no teratogenic effect. Slight foetal toxicity was manifested by decreased foetal weight (day 3 of gestation, 6 mg/kg) and(More)
The effects of three heavy metal cations, mercury (II), copper (II), and chromium (VI), on the growth of the rumen ciliate Entodinium caudatum in vitro culture was studied. The E. caudatum culture was challenged by HgCl2, CuCl2, and K2Cr2O7 for a period of 4 days. The tested concentrations of mercury (II) and copper (II) were 1, 5, 10, 20, 50 mg/L and 2,(More)
AIMS Enrichment of wheat bran (WB), corn meal (CM) and barley flakes (BF) with the oleaginous fungus Cunninghamella echinulata (CE) might lead to effective use of these by-products in ruminant nutrition. We examined their effects on rumen fermentation and lipid metabolism. METHODS AND RESULTS WB, CM and BF substrates without or with brewer's grains (WBG,(More)
  • 1