Katarína Michalková

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The CD9 cell surface molecule has been found to be important for the fertilization process of mammals. The aim of this study was to investigate, whether the molecule CD9 is expressed on bull sperm during the spermatogenesis and maturation of spermatozoa as well as in bull reproductive organs and their secreta. The expression of bovine CD9 was examined by(More)
The bovine maturation-associated sperm membrane antigen CD52-like molecule has been analysed using a mouse anti-sperm monoclonal antibody developed against bull spermatozoa. The antigen recognised by monoclonal antibody IVA-543 was detected on blood mononuclear cells (including lymphocytes and monocytes) and on a minor population of polymorphonuclear(More)
Fertilization process is a very clever and unique process comprising some essential steps resulting in formation of zygote. Tetraspanin CD9 is considered to be a serious candidate molecule participating in these events. The importance of CD9 has been discussed in relation to acrosome reaction, sperm-binding, sperm-penetration, sperm-egg fusion and(More)
Sperm-egg interaction and fusion represent a key moment of fertilization. In mammals, it is not possible without the interaction of the tetraspanin superfamily proteins including CD81. A detailed immunohistochemical localization of CD81 was monitored in bovine oocytes during different maturation stages, as well as during early embryogenesis. In addition,(More)
The objective of this research was to study the expression of cell membrane molecules CD9 and CD41/61 of transgenic rabbit with integrated human factor VIII (rhFVIII) gene construct. The expressions of these molecules have been monitored during two lactations of transgenic rabbits and simultaneously compared with the expression of the same molecules of(More)
Tetraspanin CD9 is one of the egg membrane proteins known to be essential in fertilization process. The presence and localization of CD9 molecule in spermatozoa and its possible function in reproduction are still unclear. In our study, we describe the localization of CD9 on bull spermatozoa. In the immunofluorescence assay, the positive signal has been(More)
Mammalian spermatozoa, produced by the testes, must undergo maturation in the epididymis to gain the ability to fertilize an ovum. This process includes structural modifications, such as changes in the lipid and protein composition of the plasma membrane. The CD81 and CD9 proteins, which are members of tetraspanin superfamily, have been found to be involved(More)
Membrane cofactor protein (CD46) is complement regulatory protein with probable function in the reproduction process. Expression of CD46 on human, mice, rat and guinea pig spermatozoa is restricted to the inner acrosomal membrane. In spite of the presence of anti-sperm antibodies and other potential complement activating agents in follicular fluid, CD46 is(More)
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