Katarína Kimáková

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BACKGROUND The species of the Hypericum genus are markedly variable in morphological, physiological and biochemical traits. They significantly differ in their area of distribution, which may determine their natural tolerance to environmental conditions, such as temperature extremes. OBJECTIVE To test the hypothesis that the species growing worldwide in(More)
Meristems of in vitro-grown Hypericum perforatum L. plants were precultured for 3, 10, or 14 days in the presence of 0.5 M mannitol, or 0.076 µM or 0.76 µM abscisic acid, in RM basal liquid culture medium supplemented with 0.5 mg/l 6-benzylaminopurine and subsequently subjected to cryopreservation by the slow freezing method. The survival rate – determined(More)
Plants of the genus Hypericum are widely known for their therapeutic properties. The most biologically active compounds of this genus are naphtodianthrones and phloroglucinols. Indirect desorption electrospray ionization mass spectrometry (DESI-MS) imaging allows visualization and localization of secondary metabolites in different plant tissues. This study(More)
Uptake of 14C-sucrose and 14C-NAA applied into MS medium supplemented with relevant growth substances in a period of four weeks, one week prior to transfer and on transferring to hormone free media was investigated using callus forming explants of tobacco (Nicotiana tabacum L.) and cabbage (Brassica oleracea L. vat. capitata) hypocotyl explants. Different(More)
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