Kasper L. Jacobsen

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Calves inoculated with Escherichia coli O157:H7 and fed either a high-roughage or high-concentrate diet were evaluated for rumen proliferation and fecal shedding of E. coli O157:H7. Calves fed the high-roughage diet had lower mean rumen volatile fatty acid concentrations and higher rumen pH values than did calves fed the high-concentrate diet. Despite these(More)
The immunotoxicologic effects of d-limonene were determined. This naturally occurring substance is widely used in food flavorings and is a common additive in cosmetics. In the present study, BALB/c mice were treated with d-limonene for 9 wk. Effects on T- and B-cell responses were determined after 4 and 8 wk of treatment. Concanavalin-A responses at 8 wk,(More)
OBJECTIVE To determine prevalence of papillomatous digital dermatitis (PDD) among culled adult dairy and beef cattle in the southeastern United States. ANIMALS 815 cattle examined during 4 visits to a slaughterhouse. PROCEDURE The left hind foot of each animal was examined for gross lesions of PDD. Breed and sex of the animals were recorded. Lesions(More)
Network Coding (NC) is a hot topic nowadays. Even though it has proven its applicability to wireless networks, there are still some questions whether network coding is feasible for small devices. To answer this question an implementation of the Partial Network Coding (PNC) buffer scheme is done on a wireless sensor and the results are described within this(More)
Transforming growth factor e (TGFe) was demonstrated immunohistochemically in the bovine mammary gland, mainly in the glandular and ductal epithelium. In the teat, its expression was largely limited to the skin keratinocytes, ductal epithelium and ductal glands. It is suggested that this growth factor plays a role in lactation.
Natural bovine leukocyte interferon (IFN) was produced in high titer (15,314 U/ml), with Sendai virus as the inducer at 300 hemagglutinating units (HA)/ml and 12 h incubation. Its antiviral activity was completely stable for 30 days at 4 degrees C, -20 degrees C, or -70 degrees C, and 96 h at 37 degrees C, but some activity was lost after 15 min at 56(More)
In a double-blind study, the commercial agar-gel immunodiffusion test (AGID) was compared with a radioimmunoprecipitation assay (RIA) performed with glycoprotein (gp) antigen for detection of antibodies to bovine leukemia virus. Of 240 sera tested, 115 were from adult cows and 125 were from precolostral calves. Most adult animals were tested within 1 week(More)
Bovine leukocyte interferon (BoL-IFN), produced in bovine peripheral blood leukocytes after priming and induction with Sendai virus, was concentrated by precipitation with KSCN (pH 3.5) and purified by gel column chromatography. Recovery of BoL-IFN from precipitation was higher when crude BoL-IFN containing more fetal bovine serum (FBS) was used. However,(More)
Holstein calves (3 to 5 months of age) were used to develop an animal model sensitive to environmental toxicants. In the present study, the fire ant toxicant AMDRO was fed (113.5 g/day/calf) to weanling castrated calves (9 test and 9 controls) for 7 weeks. As early as 14 days after the start of the AMDRO feeding, leukopenia was observed. Differential counts(More)
Various methods for removal or inactivation of Sendai inducing virus from natural bovine leukocyte interferon (IFN) preparations were compared and the effects of Sendai virus in microtiter cytopathic effect (CPE) inhibition assay were investigated. Ultraviolet light and ultracentrifugation at either 108,000 X g or 145,000 X g for 2 h proved to be inadequate(More)