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The overexpression of recombinant proteins in microorganisms may lead to a metabolic depression or collapse of the cell factory. In order to understand this process and to optimize the cellular productivity the stress response was investigated. The expression of the recombinant human superoxide dismutase (SOD) was induced under steady state conditions and(More)
A major challenge in microarray design is the selection of highly specific oligonucleotide probes for all targeted genes of interest, while maintaining thermodynamic uniformity at the hybridization temperature. We introduce a novel microarray design framework (Thermodynamic Model-based Oligo Design Optimizer, TherMODO) that for the first time incorporates a(More)
BACKGROUND In the biopharmaceutical industry, Escherichia coli (E. coli) strains are among the most frequently used bacterial hosts for producing recombinant proteins because they allow a simple process set-up and they are Food and Drug Administration (FDA)-approved for human applications. Widespread use of E. coli in biotechnology has led to the(More)
The advancement of bioprocess monitoring will play a crucial role to meet the future requirements of bioprocess technology. Major issues are the acceleration of process development to reduce the time to the market and to ensure optimal exploitation of the cell factory and further to cope with the requirements of the Process Analytical Technology initiative.(More)
We describe a prokaryotic expression system using the autoproteolytic function of N(pro) from classical swine fever virus. Proteins or peptides expressed as N(pro) fusions are deposited as inclusion bodies. On in vitro refolding by switching from chaotropic to kosmotropic conditions, the fusion partner is released from the C-terminal end of the autoprotease(More)
Escherichia coli K-12 and B strains are among the most frequently used bacterial hosts for production of recombinant proteins on an industrial scale. To improve existing processes and to accelerate bioprocess development, we performed a detailed host analysis. We investigated the different behaviors of the E. coli production strains BL21, RV308, and HMS174(More)
We consider the problem of visually servoing a legged vehicle with unicycle-like nonholonomic constraints subject to second-order fore-aft dynamics in its horizontal-plane. We target applications to rugged environments characterized by complex terrain likely to significantly perturb the robot's nominal dynamics. At the same time, it is crucial that the(More)
BACKGROUND Interpretation of comprehensive DNA microarray data sets is a challenging task for biologists and process engineers where scientific assistance of statistics and bioinformatics is essential. Interdisciplinary cooperation and concerted development of software-tools for simplified and accelerated data analysis and interpretation is the key to(More)
OBJECTIVES Recombinant protein production processes in Escherichia coli are usually operated in fed-batch mode; therefore, the elaboration of a fed-batch cultivation protocol in microtiter plates that allows for screening under production like conditions is particularly appealing. RESULTS A highly reproducible fed-batch like microtiter plate cultivation(More)
Self-sufficiency in robotics motivates the need for continual improvement in autonomous navigation for mobile robots. Wall-following provides a simplification of 2-D navigation problems by providing a reference by which these problems are reduced to 1-D. A laser scanner for wall detection (abstracted as an active tangent/curvature sensor) is used on a(More)
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