Karina Gershkovich

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Kinetic parameters (k(Pg) and K(Pg)) were determined for activation of Glu-plasminogen (Glu-Pg) and Lys-plasminogen (Lys-Pg) type I (with N-linked carbohydrate chain at Asn-289) and type II (with unsubstituted Asn-289) by plasmin-staphylokinase (Pm-STA) complex. The K(Pg) values for Glu-Pg I and Lys-Pg I (17.1 and 11.2 microM, respectively) were higher than(More)
Thrombolytic therapy with high doses of streptokinase (SK), which are required due to its rapid clearance from the bloodstream, is accompanied by side effects. In this work, the SK was incorporated into water-soluble polyethylene glycol (PEG) microcapsules with the double emulsification method in order to increase its lifetime in bloodstream and decrease(More)
Three-dimensional organization of intermediate soluble forms of fibrin-polymers--a product of fibrin-monomer assembly--in the presence of non-denaturating urea concentrations has been studied. Hydrodynamic parameters of fibrinogen and fibrin-polymers were obtained by viscosimetry, dynamic light scattering and analytic ultracentrifugation. Using Yamakawa's(More)
In this study we have produced for the first time a native fibrinogen copolymer with a fragment of fibrin E. and the molecular mechanism of its formation was studied by different physicochemical methods. Based on the data of angular dependency of the Debay scattering factor, the average molecular mass, coefficients of translational diffusion and the(More)
INTRODUCTION The binding of plasminogen (Pg) to cell receptors and extracellular ligands facilitates its activation to plasmin, which stimulates the extracellular matrix degradation, neoangiogenesis and tumor invasion. Plasmin can also degrade IgG thereby exposing C-terminal lysine residues. Previously, we have found IgG specifically bounded to Pg in the(More)
The frequency of venous and arterial thromboses and plasminogen level were investigated in 78 patients with antiphospholipid syndrome (APS), 35 of whom with systemic lupus erythematosus (SLE+APS) and 43 - with primary APS (PAPS). The levels and genotype of plasminogen activator inhibitor type 1 (PAI-1) were determined in 45 patients with APS, of whom 21(More)
The methods of viscosimetry, the Rayleigh light-scattering and analytical ultracentrifugation were applied to study the physicochemical mechanism of the effect of fragment D on the structure of fibrin equilibrium oligomers. Using the values of intrinsic viscosity, weight average molecular masses and mass/length ratio it was shown that when producing an(More)
Thrombolytic therapy by high doses of streptokinase (SK) that are stipulated by its rapid clearance is accompanied by side effects. In this work for the purpose of lifetime prolongation in bloodstream and decrease in side effects SK was included in microcapsules from water-soluble polyethyleneglygol (PEG) using the double emulsification method. By variation(More)
The morphology of equilibrium of soluble fibrin oligomers at different stages of assembly was studied. Results of Rauleigh's light scattering, analytical ultracentrifugation and viscosimetry show that fibrin-polymers throughout the entire homology range present rigid, rod-like structures dispersed by weight and dimensions. It was shown, that along with the(More)
Generalized concepts of some structural peculiarities of fibrinogen, its transformation into fibrin and assembly have been considered on the basis of author's and published data. The role of local conformational changes in different areas of fibrinogen molecule and of separate reaction centers in formation of single- and double-stranded rod-like equilibrium(More)