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Nicotinic acid adenine dinucleotide phosphate (NAADP) is the most potent Ca(2+)-releasing second messenger known to date. Here, we report a new role for NAADP in arrhythmogenic Ca(2+) release in cardiac myocytes evoked by β-adrenergic stimulation. Infusion of NAADP into intact cardiac myocytes induced global Ca(2+) signals sensitive to inhibitors of both(More)
Cyclic ADP-ribose (cADPR, 1, Scheme 1), originally isolated from sea urchins by Lee and co-workers, [1] is a general mediator of intracellular Ca 2+ ion signaling. [2] Analogues of cADPR have been extensively designed and synthesized [3, 4] because of their potential usefulness for investigating the mechanisms of cADPR-mediated Ca 2+ release and application(More)
Two nicotinamide adenine dinucleotide (NAD(+)) analogues modified at the 6 position of the purine ring were synthesized, and their substrate properties toward Aplysia californica ADP-ribosyl cyclase were investigated. 6-N-Methyl NAD(+) (6-N-methyl nicotinamide adenosine 5'-dinucleotide 10) hydrolyzes to give the linear 6-N-methyl ADPR (adenosine(More)
Tubulin and the two microtubule-associated proteins MAP2 and tau were purified from pig brain. The ability of MAP2 and tau to induce tubulin polymerization in the presence of guanyl-5'-yl methylene diphosphonate was compared in parallel experiments. MAP2 at 0.2 mol/mol of tubulin induced the polymerization of tubulin exclusively into ribbons 0.45 +/- 0.05(More)
ii To my wife, Genevieve, for her dedication and example of perseverance. iii ACKNOWLEDGEMENTS I am grateful to all members of my thesis committee, ProfessorGasparoni for their support, guidance and patience. I am indebted to them for going out of their way to make completion of this endeavor possible. I would also be remiss if I did not give thanks to Dr.(More)
Here we describe the successful synthesis of cyclic ADP-4-thioribose (cADPtR, 3), designed as a stable mimic of cyclic ADP-ribose (cADPR, 1), a Ca(2+)-mobilizing second messenger, in which the key N1-β-thioribosyladenosine structure was stereoselectively constructed by condensation between the imidazole nucleoside derivative 8 and the 4-thioribosylamine 7(More)
Note The contents of this publication do not necessarily reflect the official opinions of the European Commission or other European Communities institutions. Neither the European Environment Agency nor any person or company acting on the behalf of the Agency is responsible for the use that may be made of the information contained in this report. All rights(More)
18 keratin genes and a surprisingly high number of pseudogenes related to keratin genes 8 and Genes for intermediate filament proteins and the draft sequence of the human genome: novel progression Keratin binding to 14-3-3 proteins modulates keratin filaments and hepatocyte mitotic abnormal function of the gastrointestinal tract, hepatobiliary system, and(More)
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