— This paper evaluates the performance of two methods , level set active contours and watershed region growing, applied to live cell image segmentation. These methods suffer from opposite problems. As the level set method tends to merge clustered cells, the watershed method often results in over-segmentation. These problems can be reduced by adding an image… (More)
This paper describes a system for in vitro cell migration analysis. Adult neural stem/progenitor cells are studied using time-lapse microscopy and thereafter stained immunohistochemically to find and distinguish between undifferentiated glial progenitor cells and cells having differentiated into type-1 or type-2 astrocytes. The cells are automatically… (More)
This is an initial study aiming to solve the problem how to segment stem cells in gray scale time-lapse microscopy images.
In this paper we present an extension to the formulation of Active Contour Models (Snakes) by including an additional contour-deforming force. The new force is derived from the optical flow field calculated between two time-consecutive images. The addition of the new force assists the snake in tracking desired dynamics while the traditional snake forces… (More)