Karim Chemlal

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BACKGROUND Mycobacterium ulcerans disease, or Buruli ulcer (BU), is an indolent, necrotizing infection of skin, subcutaneous tissue and, occasionally, bones. It is the third most common human mycobacteriosis worldwide, after tuberculosis and leprosy. There is evidence that M. ulcerans is an environmental pathogen transmitted to humans from aquatic niches;(More)
The purpose of this study was to develop a simple procedure for cell lysis and DNA extraction for direct detection of Mycobacterium ulcerans in aquatic insects, gills and intestinal contents of fish, molluscs and human tissue samples using a nested PCR method specific for the insertion sequence IS2404. The simultaneous action of sodium N-lauroyl sarcosine,(More)
This study reports the existence of phospholipase C and D enzymatic activities in Mycobacterium ulcerans cultures as determined by use of thin-layer chromatography to detect diglycerides in hydrolysates of radiolabeled phosphatidylcholine. M. ulcerans DNA sequences homologous to the genes encoding phospholipase C in Mycobacterium tuberculosis and(More)
A 63 year-old previously healthy man presented with marked lymphocytosis. Physical examination showed splenomegaly but no adenopathy. The blood count was 38.5ϫ10 9 leuko-cytes with 88% lymphoid cells, 170 g/L hemoglobin and 246ϫ10 9 /L platelets. The lymphoid cells were large with a single thick-rimmed nucleolus in a round regular nucleus. The chromatin was(More)
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