Karijn P. M. Suijkerbuijk

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TWIST1, an antiapoptotic and prometastatic transcription factor, is overexpressed in many epithelial cancers including breast. Only little is known regarding the regulation of TWIST1 in these cancers. Recently, an increase in the TWIST1 promoter methylation has been shown in breast cancers. To correlate the percentage of TWIST1 promoter methylation to the(More)
The need for additional breast cancer screening tools is indisputably high, as one may conclude from the high rate of interval malignancies in women undergoing regular screening. DNA promoter methylation frequently occurs during breast carcinogenesis and is an early event in this process. Moreover, a field defect for methylation has been described and(More)
BACKGROUND Promoter hypermethylation has emerged as a promising cancer biomarker. Currently, a large variety of quantitative and non-quantitative techniques is used to measure methylation in clinical specimens. Here we directly compared three commonly used methylation assays and assessed the influence of tissue fixation, target sequence location and the(More)
The high rate of interval malignancies urges for new screening methods for women at high risk for breast cancer. Nipple aspiration provides direct access to the breast tissue and its DNA, and therefore is a likely candidate, but clinical applications have been limited by the failure to obtain nipple aspiration fluid from most women. We performed(More)
Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 (BNIP3) is a pro-apoptotic member of the Bcl-2 family induced under hypoxia [3,5,6]. Low or absent expression has been described in several human tumors, resulting in poor prognosis [1]. We previously reported that BNIP3 expression is lost in a significant portion of invasive breast cancers, which was(More)
Defects in DNA that activate oncogenes or inactivate tumour-suppressor genes are regarded as a crucial step in tumour development. Understanding the processes that modulate gene activity, the so-called epigenetic processes, is gaining importance in the search for factors responsible for uncontrolled cell growth. Cell proliferation is determined by(More)
Breast cancer is the leading cause of cancer death in women in the Western world. In the Netherlands, the incidence is about 12,000 per year, which means that eventually every 9 th woman will get breast cancer. The most wellestablished breast cancer risk factor is the presence of a germline mutation in the BRCA1 or BRCA2 genes, which indicates a life time(More)
INTRODUCTION Nipple fluid aspiration provides direct non-invasive sampling of fluid from the mammary ductal system, where the majority of breast cancers originate. DNA promoter hypermethylation ("methylation") occurs early and at high frequency in breast carcinogenesis, bearing the potential as a biomarker for cancer detection at its earliest stages. We(More)
BACKGROUND Despite intensive surveillance, a high rate of interval malignancies is still seen in women at increased breast cancer risk. Therefore, novel screening modalities aiming at early detection remain needed. The intraductal approach offers the possibility to directly sample fluid containing cells, DNA and proteins from the mammary ductal system(More)
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