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Refinement of Tools for Targeted Gene Expression in Drosophila
TLDR
The increased strength and reliability of these optimized reagents overcome many of the previous limitations of these methods and will facilitate genetic manipulations of greater complexity and sophistication in Drosophila melanogaster.
The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
TLDR
The various tools developed and the status of the TRiP collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes, are described.
Genetic Reagents for Making Split-GAL4 Lines in Drosophila
TLDR
This report describes a set of over 2800 transgenic lines for use with the split-GAL4 intersectional method, in which expression of the transgene only occurs where two different enhancers overlap in their expression patterns, to achieve the desired specificity.
A Role for the Cytoplasmic DEAD Box Helicase Dbp21E2 in Rhodopsin Maturation and Photoreceptor Viability
TLDR
Analysis of a green fluorescent protein (GFP)-tagged Rh1 rhodopsin construct placed under control of a heat shock promoter showed that Dbp21E21 fails to efficiently transport Rh1 from the photoreceptor cell body to the rhabdomere.
Genetic reagents for making split-GAL4 lines in Drosophila
TLDR
This report describes a set of over 2,800 transgenic lines for use with the split-GAL4 intersectional method, which can be used to reproducibly target expression of transgenes to small, defined subsets of cells.
Probing Synaptic Transmission and Behavior in Drosophila with Optogenetics: A Laboratory Exercise.
TLDR
These modules introduced educators and students to the use of optogenetic stimulation to control behavior and evoked release at a model synapse, and establish a basis for students to explore neurophysiology using this technique, through recapitulating classic experiments and conducting independent research.
Hierarchical architecture of dopaminergic circuits enables second-order conditioning in Drosophila
TLDR
This work identifies a feedforward circuit formed between dopamine subsystems and shows that it is essential for second-order conditioning, an ethologically important form of higher-order associative learning.