Karen C. Dwyer

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Langerhans cell histiocytosis (LCH) is a myeloproliferative disorder characterized by lesions composed of pathological CD207(+) dendritic cells with an inflammatory infiltrate. BRAFV600E remains the only recurrent mutation reported in LCH. In order to evaluate the spectrum of somatic mutations in LCH, whole exome sequencing was performed on matched LCH and(More)
Immune evasion is an emerging hallmark of cancer progression. However, functional studies to understand the role of myeloid-derived suppressor cells (MDSCs) in the tumor microenvironment are limited by the lack of available specific cell surface markers. We adapted a competitive peptide phage display platform to identify candidate peptides binding MDSCs(More)
To enhance the therapeutic index of allogeneic hematopoietic SCT (HSCT), we immunized 10 HLA-matched sibling donors before stem cell collection with recipient-derived clonal myeloma Ig, idiotype (Id), as a tumor antigen, conjugated with keyhole limpet hemocyanin (KLH). Vaccinations were safe in donors and recipients. Donor-derived KLH- and Id-specific(More)
Granulocytes play a key role in innate immunity and the most common functional assays are phagocytosis and oxidative burst. The purpose of this technical note is to use image-based flow cytometry to divide activated granulocytes into unique subsets based on their degree of phagocytosis and oxidative burst in response to different experimental incubations.(More)
Molecular and immune heterogeneity in synchronous melanoma metastases Alexandre Reuben, Christine Spencer, Jason Roszik, John Miller, Lawrence Kwong, Hong Jiang, Cara Haymaker, Pei-Ling Chen, Jacob Austin-Breneman, Whijae Roh, Latasha Little, Yu Cao, Haven Garber, Marie-Andrée Forget, Vancheswaran Gopalakrishnan, Rodabe Amaria, Michael Davies, Chantale(More)
Cancer immune evasion is an emerging hallmark of disease progression. Functional studies to understand the role of myeloid-derived suppressor cells (MDSC) in the tumor microenvironment however, are limited by the lack of available specific cell surface markers. We adapted a competitive peptide phage display platform to identify candidate peptides binding(More)
There have been significant advances in the treatment of melanoma via the use of targeted therapy and immunotherapy, however a significant proportion of patients still progress on treatment. Intense research efforts are underway to better understand resistance, and multiple molecular resistance mechanisms to targeted therapy have been identified. There is a(More)
Background Oncolytic viruses are promising alternative cancer therapies that can cause anti-cancer immunity. We found Delta-24-RGD, an oncolytic adenovirus currently undergoing Phase I clinical trial in patients with recurrent glioblastomas, induced anti-glioma immunity in an immunpcompetent mouse model. We hypothesized that the anti-cancer immunity(More)
Gheath Alatrash1,*, Haven R. Garber#1, Mao Zhang#1, Pariya Sukhumalchandra1, Yihua Qiu2, Haroon Jakher1, Alexander A. Perakis1, Lisa Becker1, Suk Young Yoo3, Karen C. Dwyer1, Kevin Coombes4, Amjad H. Talukder5, Lisa S. St John1, Vladimir Senyukov6, Dean A. Lee6, Anna Sergeeva1, Hong He1, Qing Ma1, Paul M. Armistead7, Jason Roszik5,8, Elizabeth A.(More)
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