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BACKGROUND Developmental, physiological and tissue engineering studies critical to the development of successful myocardial regeneration therapies require new ways to effectively visualize and isolate large numbers of fluorescently labeled, functional cardiomyocytes. METHODOLOGY/PRINCIPAL FINDINGS Here we describe methods for the clonal expansion of(More)
Various types of cardiomyocytes undergo changes in automaticity and electrical properties during fetal heart development. Human embryonic stem cell-derived cardiomyocytes (hESC-CMs), like fetal cardiomyocytes, are electrophysiologically immature and exhibit automaticity. We used hESC-CMs to investigate developmental changes in mechanisms of automaticity and(More)
The maturation of cardiac myocytes during the immediate prenatal period coincides with changes in the mechanical properties of the extracellular matrix. We investigated the effects of extracellular stiffness on cardiomyocyte maturation in neonatal rat ventricular myocytes grown on collagen-coated gels. Cells on 10-kPa substrates developed aligned(More)
We have developed a novel wet extrusion process to fabricate nonwoven self-assembled microfiber scaffolds with uniform diameters less than 5 μm and without any postmanipulation. In this method, a poly(L-lactic acid) solution flows dropwise into a stirring nonsolvent bath, deforming into liquid polymer streams that self-assemble into a nonwoven microfiber(More)
Nano-Y2O3:Eu3+ was synthesized with oxalic acid as precipitate agent while adding little surfactant controlling the condition of the reaction. We obtained nanoscale powder. The particle diameter was 15-19 nm. The agglomerate size was less 1.0 micron. Compared with the ordinary powder of Y2O3:Eu3+, the peak of emission spectrum of nano-Y2O3:Eu3+ is at lambda(More)
We previously showed that hyperthermia induced in rhesus monkeys (Macaca mulatta) by forced passive heating "primes" the peripheral lymphocyte population for increased synthesis of interferon-gamma (IFN-gamma). It was not clear whether these data could be extrapolated to the physiological response in naturally occurring fever. Therefore, in the current(More)
The spectral properties of nanocrystallines Dy0.5Sr0.5CoO3-Y were studied. The results show that the band edge of emission spectra is blue shift when the size of particles decrease to nanometer. The infrared absorption peak fission of nanocrystallines and the XRD diffraction peak of nanocrystallines were widen than microcrystallines. It was appeared as(More)
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