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1. Oxytocin is known to act on autoreceptors of oxytocin neurones in the supraoptic nucleus (SON). We investigated whether oxytocin modulates putative oxytocin neurones by suppressing the GABAA receptor-mediated synaptic inputs on these cells. 2. GABAergic inhibitory postsynaptic currents (IPSCs) were recorded from SON neurones in hypothalamic slices from(More)
There is accumulating evidence that glial cells actively modulate neuronal synaptic transmission. We identified a glia-derived soluble acetylcholine-binding protein (AChBP), which is a naturally occurring analogue of the ligand-binding domains of the nicotinic acetylcholine receptors (nAChRs). Like the nAChRs, it assembles into a homopentamer with(More)
Molluscan neurons and muscle cells express transient (T-type like) and sustained LVA calcium channels, as well as transient and sustained HVA channels. In addition weakly voltage sensitive calcium channels are observed. In a number of cases toxin or dihydropyridine sensitivity justifies classification of the HVA currents in L, N or P-type categories. In(More)
1. GABAA receptor-mediated synaptic innervation of oxytocin neurones in the supraoptic nucleus (SON) was analysed in adult female rats going through their first reproductive cycle by recording the spontaneous inhibitory postsynaptic currents (sIPSCs) at six stages of female reproduction. 2. During pregnancy we observed a reduction in the interval between(More)
We describe here the electrophysiological characterization of a dual inhibitory action of FMRFamide (FMRFa, Phe-Met-Arg-Phe-NH2) on the caudodorsal cells (CDCs) of the pond snail Lymnaea stagnalis: (i) a transient hyperpolarizing response (H-response) and (ii) a suppression of the excitability of the cells, which lasted as long as the peptide was present.(More)
The light green cells (LGCs) in the central nervous system of the pond snail Lymnaea stagnalis form a homogeneous group of neuroendocrine cells that are involved in the control of growth and metabolism. These cells are inhibited by dopamine and the neuropeptides APGWamide, FMRFamide and GGSLFRFamide. Thus, the LGCs form an endogenous system in which(More)
Structure activity relations (SAR) of FMRFa on the transient hyperpolarizing response and long lasting depression of excitability of neurosecretory caudo dorsal cells (CDCs) of the pond snail Lymnaea stagnalis were examined. Although these effects to FMRFa occur independently, the SARs for the induction of both responses were identical suggesting that CDCs(More)
1. Whole-cell voltage clamp recordings were made from 141 rat pituitary melanotropic cells in short-term, serum-free, primary culture. The effects of the dopamine D2 receptor agonist, LY 171555, on sodium, potassium and barium currents were investigated. 2. Application of 1 microM-LY 171555 did not affect the inward sodium and outward potassium currents. 3.(More)
Dopamine D2 receptor stimulation inhibited high-threshold, slowly inactivating (L-type) barium currents of isolated, rat pituitary melanotropes in primary culture. The extent of inhibition depended on the concentration of LY 171555 applied. Current activation in the presence of LY 171555 was described by two time constants, a fast one, also observed under(More)
The in vitro release of acetylcholine in rat brain tissue was inhibited by 9-amino-1,2,3,4-tetrahydroacridine (THA). Atropine antagonized this effect of THA. As THA does not display an affinity for muscarinic receptors, we conclude that THA inhibits acetylcholinesterase activity. In electrophysiological studies with neurons of Lymnaea stagnalis, THA(More)