Kar Man Leung

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Therapeutic recombinant monoclonal antibodies (mAbs) are commonly produced by high-expressing, clonal, mammalian cells. Creation of these clones for manufacturing remains heavily reliant on stringent selection and gene amplification, which in turn can lead to genetic instability, variable expression, product heterogeneity and prolonged development(More)
Background Cell line generation (CLG) in the scope of bioproduc-tion can be defined as a method to isolate a single cell expressing a recombinant protein of interest. The standard method of CLG often involves the introduction of the transgene into a cell in an attempt to use the cellular machinery for transcription, translation and secretion. The use of(More)
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