Kannan Thirumalai Raj

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A multiplex polymerase chain reaction (MPCR)-based assay was developed for the simultaneous detection of Vibrios using the genus-specific RNA polymerase subunit A (rpoA) gene and specific detection of toxin-producing Vibrio cholerae strains using two sets of primer based on cholera toxin subunit A (ctxA) and repeat in toxin subunit A (RtxA)-producing genes.(More)
A multiplex PCR (MPCR) assay was developed for the detection of Staphylococcus aureus using selected primers designated on the genus-specific gap gene, species-specific nuc gene and enterotoxin-producing EntC1 gene. The internal regions amplified had a product size of 933 bp, 273 bp and 531 bp, respectively. This MPCR assay had a sensitivity to detect 10(More)
Hemolytic strains of Aeromonas spp. from fish and fishery products were detected by multiplex PCR. The selected primers for the amplification of segments of ahh1, asa1 and 16S rRNA gene yielded products with the size of 130 bp, 249 bp and 356 bp, respectively. This assay was found to be highly sensitive, as it could detect 7 and 9 cells of Aeromonas(More)
A rapid and sensitive multiplex PCR (MPCR)-based assay was developed for the detection of Salmonella serovars in shrimps within 8 h of pre-enrichment. Five sets of primers from different genomic sequences such as fimA, himA, hns, invA and hto genes were selected for the detection of serovars of Salmonella enterica such as S. Typhi, S. Paratyphi A, S.(More)
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