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A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing
TLDR
A virus-based guide RNA (gRNA) delivery system for CRISPR/Cas9 mediated plant genome editing (VIGE) that can be used to precisely target genome locations and cause mutations is reported. Expand
Progress and prospects in plant genome editing
TLDR
The current status of plant genome editing is described, focusing on newly developed genome editing tools and methods and their potential applications in plants. Expand
MYB75 Phosphorylation by MPK4 Is Required for Light-Induced Anthocyanin Accumulation in Arabidopsis[OPEN]
TLDR
It is shown that MPK4 phosphorylation of MYB75 increases its stability and is essential for light-induced anthocyanin accumulation, revealing an important role for a MAPK pathway in light signal transduction. Expand
Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins
TLDR
DNA-free editing of bread wheat is described by delivering in vitro transcripts (IVTs) or ribonucleoprotein complexes (RNPs) of CRISPR/Cas9 by particle bombardment as an extension of the previously published protocol on genome editing in bread wheat. Expand
Virus-Based MicroRNA Silencing in Plants1[C][W][OPEN]
TLDR
It is demonstrated that tobacco rattle virus-based VbMS is a powerful tool to silence endogenous miRNAs and to dissect their functions in different plant species. Expand
An improved method for chloroplast genome sequencing in non-model forest tree species
TLDR
A modified method for the isolation of intact chloroplasts is reported, which needs less than 0.5 g leaf material and can be applied to the reconstruction of chloroplast genomes for plant evolutionary studies, especially in non-model tree species. Expand
Genome-Wide Characterization of miRNAs Involved in N Gene-Mediated Immunity in Response to Tobacco Mosaic Virus in Nicotiana benthamiana
TLDR
This study reports an efficient method using high-throughput sequencing and bioinformatics to identify genome-wide miRNAs in N. benthamiana and provides a framework for further analysis of miRNA functions in plant immunity. Expand
Engineer complete resistance to Cotton Leaf Curl Multan virus by the CRISPR/Cas9 system in Nicotiana benthamiana
TLDR
The transgenic plants expressing Cas9 and dual gRNAs that target different regions of the CLCuMuV genome confer complete resistance to virus infection, thereby demonstrating a novel approach for engineering resistance to geminiviruses. Expand
Complete chloroplast genome of the Oriental white oak: Quercus aliena Blume
TLDR
Border analysis of cp genome of Quercus aliena and other 10 most closely related cp genomes revealed that most Fagaceae species have high similarity either in structure or distribution of these genes except for Trigonobalanus doichangensis. Expand
An efficient Potato virus X -based microRNA silencing in Nicotiana benthamiana
TLDR
This study shows the Potato virus X (PVX)-based TM expression causes strong miRNA silencing in Nicotiana benthamiana, and a PVX-based VbMS is a powerful method to study miRNA function and may be useful for high-throughput investigation of mi RNA function in N.benthamiana. Expand
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