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AtCDC5 regulates the G2 to M transition of the cell cycle and is critical for the function of Arabidopsis shoot apical meristem
- Zhiqiang Lin, Kangquan Yin, Dan-dan Zhu, Zhang-liang Chen, H. Gu, L. Qu
- Biology, Environmental ScienceCell Research
- 1 September 2007
The functional characterization of the CDC5 gene in Arabidopsis thaliana is reported, suggesting that AtCDC5 is essential for the G2/M phase transition and may regulate the function of SAM by controlling the expression of STM and WUS.
A high-throughput screening system for Arabidopsis transcription factors and its application to Med25-dependent transcriptional regulation.
A high-throughput Y1H/Y2H screening system with a Gateway-compatible Gal4-AD-TF library of 1589 Arabidopsis TFs is developed, finding that three of these TFs, all from the AP2-EREBP family, interact directly both with Med25 and the GCC-box of PDF1.2, suggesting that Med25 regulates PDF 1.2 expression through these three TFs.
A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing
A virus-based guide RNA (gRNA) delivery system for CRISPR/Cas9 mediated plant genome editing (VIGE) that can be used to precisely target genome locations and cause mutations is reported.
MYB75 Phosphorylation by MPK4 Is Required for Light-Induced Anthocyanin Accumulation in Arabidopsis[OPEN]
It is shown that MPK4 phosphorylation of MYB75 increases its stability and is essential for light-induced anthocyanin accumulation, revealing an important role for a MAPK pathway in light signal transduction.
Arabidopsis RAP2.2 plays an important role in plant resistance to Botrytis cinerea and ethylene responses.
It is demonstrated that RAP2.2 functions as an important regulator in Botrytis resistance and ethylene responses and its role in the ethylene signaling pathway is assessed by molecular and genetic approaches.
Progress and prospects in plant genome editing
The current status of plant genome editing is described, focusing on newly developed genome editing tools and methods and their potential applications in plants.
An improved method for chloroplast genome sequencing in non-model forest tree species
- F. Du, T. Lang, Sihai Lu, Yuyao Wang, Junqing Li, Kangquan Yin
- BiologyTree Genetics & Genomes
- 9 October 2015
A modified method for the isolation of intact chloroplasts is reported, which needs less than 0.5 g leaf material and can be applied to the reconstruction of chloroplast genomes for plant evolutionary studies, especially in non-model tree species.
Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins
DNA-free editing of bread wheat is described by delivering in vitro transcripts (IVTs) or ribonucleoprotein complexes (RNPs) of CRISPR/Cas9 by particle bombardment as an extension of the previously published protocol on genome editing in bread wheat.
Virus-Based MicroRNA Silencing in Plants1[C][W][OPEN]
It is demonstrated that tobacco rattle virus-based VbMS is a powerful tool to silence endogenous miRNAs and to dissect their functions in different plant species.
Genome editing for plant disease resistance: applications and perspectives
The challenges for breeding resistant crops and applications of genome editing technology in generating plants with resistance to bacterial, fungal and viral diseases are summarized.