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☆ We would like to thank our two anonymous refer improved the paper. We would also like to thank Henning Chen, Igor Livshits, Hui He,Marek Kapicka, Tee Kilenthong Mehta, Cheng-ZhongQin, Peter Rupert, Guillaume Vanden seminar participants from a number of different venues fo our own. ⁎ Corresponding author at: Department of Economics California, Santa(More)
Ovarian cancer is the number one cause of death from gynecologic malignancy. A defective p53 pathway is a hallmark of ovarian carcinoma. The p53 mutation correlates significantly with resistance to platinum-based chemotherapy, early relapse and shortened overall survival in ovarian cancer patients. PUMA (p53 upregulated modulator of apoptosis), a BH3-only(More)
OBJECTIVE We aimed to investigate the role of the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) signaling pathway and its negative feedback factor, phosphatase and tensin homolog (PTEN), in the pathogenesis of Crohn's disease (CD). METHODS Peripheral blood was collected from patients with CD and healthy(More)
PNAS-4, a novel pro-apoptotic gene, was activated during the early response to DNA damage. Previous studies have shown that hPNAS-4 can inhibit tumor growth when over-expressed in ovarian cancer cells. However, the underlying action mechanism remains elusive. In this work, we found that hPNAS-4 expression was significantly increased in SKOV3 cells when(More)
Ovarian cancer is the most common cause of death from gynecologic malignancy. Deregulation of p53 and/or p73-associated apoptotic pathways contribute to the platinum-based resistance in ovarian cancer. NOXA, a pro-apoptotic BH3-only protein, is identified as a transcription target of p53 and/or p73. In this study, we found that genetic variants of Bcl-2(More)
AIM To construct the ureI and ctB-ureI eukaryotic expression plasmid with pIRES-RD2 and study the expressing characters of those plasmids in HEK 293. METHODS We got the ureI and ctB-ureI gene from the procaryotic expression plasmid pET32a(+)-ureI and pET32a(+)-ctB-ureI by restriction enzyme BglII and XhoI, respectively.The pET32a(+)-ctB-ureI had been(More)
A label-free and selective aptamer beacon-based Love-wave biosensing device was developed for prostate specific antigen (PSA) detection. The device consists of the following parts: LiTaO3 substrate with SiO2 film as wave guide layer, two set of inter-digital transducers (IDT), gold film for immobilization of the biorecongniton layer and a(More)
Secondary pneumonia due to Staphylococcus aureus (S. aureus) causes significant morbidity and mortality. The aim of the research was designed a novel DNA vaccine encoding the Mycobacterium tuberculosis secreted antigen Ag85A fused with the influenza A virus (IAV) HA2 protein to provide protection against both influenza and secondary infection with S.(More)
Noxa is an important proapoptotic protein in the intrinsic pathway of cell apoptosis. Experiments were carried out to investigate whether Noxa could, therefore, enhance the cytotoxic effect of gemcitabine in human ovarian cancer cell lines (A2780 and COC1). In this study, the combined treatment of Noxa and gemcitabine, in vitro, significantly inhibited the(More)
OBJECTIVE To clone a novel apoptosis-related gene, human PNAS-4, and to get it expressed in E. coli. METHODS Human PNAS-4 gene was amplified by RT-PCR form A549 human lung adenocarcinoma cells and inserted into pGEX-6P-1 vector. The resulting recombinant plasmid was transformed into E. coli. BL21. Human PNAS-4 protein was expressed with IPTG induction and(More)