Oocytes arrested in the G2 phase of the cell cycle contain a p34cdc2/cyclin B complex which is kept in an inactive form by phosphorylation of its p34cdc2 subunit on tyrosine, threonine and perhaps serine residues. The phosphatase(s) involved in p34cdc2 dephosphorylation is unknown, but the product of the fission yeast cdc25+ gene, and its homologues in… (More)
Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based… (More)
Development of protein labeling techniques with small molecules is enthralling because this method brings promises for triumph over the limitations of fluorescent proteins in live cell imaging. This technology deals with the functionalization of proteins with small molecules and is anticipated to facilitate the expansion of various protein assay methods. A… (More)
Two cryptand molecules are connected via p-xyloyl, benzene-1,4-dicarbolyol and 9,10-dimethylene anthracene. Each cryptand is further derivatized with fluorophores such that electronic absorption of one fluorophore overlaps emission of the other. This way, three different systems L(1), L(2) and L(3) have been synthesized to get a better view of the effect on… (More)
A novel hybrid of azacrown ether-oxabridged fluorogenic chemosensor (1) has been synthesized: the metal ion binding ability of the hybrid molecule shows higher affinity towards transition metal ion Zn(2+) and heavy metal ion Hg(2+) compared to the parent azacrown ether which shows poor metal ion selectivity: the binding modes of the Zn(2+) and Na(+) towards… (More)
The movement of a copper ion inside the cavity of cryptands having two distinct metal binding sites at each end is linked to a fluorescence ON-OFF process in a reversible manner, providing a new way of fluorescence switching.