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Extraction of Furcellaria lumbricalis by potassium hydroxide solution shows that the maximum gel strength of polysaccharides separated is attained by extraction at concentration 0.16 M KOH. Crude extract from this algal species has a complicated monosaccharide composition; a drastic increase of gel strength by alkaline extraction of the polysaccharide(More)
The main isolation procedures (extraction, alkali modification, and precipitation) of hybrid carrageenan blend from seaweeds of the Kassari algal stratum (the Baltic Sea, Estonia) were quantitatively investigated. In the case of all extracting agents (water, KOH and NaOH solutions) used, a sharp rise occurred in the extracting rate during the first 2 h.(More)
Sol-gel transition processes of algal galactans were studied using cryofixation method in combination with freeze-drying and scanning electron microscopy (SEM) techniques. The structures formed in successive stages of gelling process upon cooling were rapidly frozen at defined temperature points and viewed by SEM. It was established that in the case of both(More)
Two different types of bioactive components of the seaweed Fucus vesiculosus were analysed: (1) polyphenols (phlorotannins) by capillary electrophoresis (CE) and (2) mineral part (including bioactive microelements) by neutron activation analysis (NAA). CE experiments were carried out using a UV detector (at 210 nm) and an uncoated silica capillary. The best(More)
Mineral composition of algal biomass from Fucus vesiculosus (the Baltic Sea, Estonia) is rich in light elements (Mg, K, Ca, Na) and halogens (Br, I). Arsenic content is also high (over 300 ppm); the content of other toxic elements (Hg, Sb, Se, etc.) is extremely low. Mineralization of the algal whole biomass does not depend on seasonal varieties. The(More)
The structure and composition of galactan from Furcellaria lumbricalis (furcellaran) were investigated in connection with rheological specificities, gel structure, and extraction conditions. The polysaccharide was characterized by 13C nuclear magnetic resonance (13C-NMR) and Fourier transform infrared spectroscopy, inductively coupled plasma-optical(More)
The effect of the number of active groups of new affinity supports--odigose and odifil (ethylsulfo-activated agarose) on the retention of the specific activity of muscle aldolase was investigated. The active center of the enzyme includes lysine able to react with activated supports. The aldolase completely retained the specific activity after immobilization(More)
The composition of varieties of Taxus growing in Estonia was analyzed by capillary electrophoresis with diode array detection (CE-DAD) for the separation of phenolic compounds, and by high performance liquid chromatography-mass spectrometry (HPLC-MS) for the determination of taxoids. The main purpose of this study was the chemotaxonomic differentiation of(More)
The composition, structure, and thermal stability of carrageenans from unattached Coccotylus truncatus (the Baltic Sea, Estonia) were investigated. The complex polysaccharide was characterized by (13)C NMR and FTIR spectroscopy, ICP-OES and gel permeation chromatography methods. The main components of C. truncatus galactan are(More)
Thermal degradation of κ-, ι-, λ-carrageenans, furcellaran, funoran and agarose samples in dry and sol states was investigated. The polysaccharides subjected to heat treatment were characterized by 1H NMR, 13C NMR and FTIR spectroscopy, size exclusion chromatography and static rheometry methods. The microstructure of galactan gels was studied using a(More)
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