Kai-tak Wan

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This paper reports the first quantitative comparison study of elastic and viscoelastic properties of oocytes from young and aged mice. A force measurement technique, including a poly(dimethylsiloxane) (PDMS) cell holding device and a sub-pixel computer vision tracking algorithm, is utilized for measuring forces applied to an oocyte and resultant cell(More)
We engineered functional cardiac patches by seeding neonatal rat cardiomyocytes onto carbon nanotube (CNT)-incorporated photo-cross-linkable gelatin methacrylate (GelMA) hydrogels. The resulting cardiac constructs showed excellent mechanical integrity and advanced electrophysiological functions. Specifically, myocardial tissues cultured on 50 μm thick(More)
Biomaterials currently used in cardiac tissue engineering have certain limitations, such as lack of electrical conductivity and appropriate mechanical properties, which are two parameters playing a key role in regulating cardiac cell behavior. Here, the myocardial tissue constructs are engineered based on reduced graphene oxide (rGO)-incorporated gelatin(More)
Microfabrication technology provides a highly versatile platform for engineering hydrogels used in biomedical applications with high-resolution control and injectability. Herein, we present a strategy of microfluidics-assisted fabrication photo-cross-linkable gelatin microgels, coupled with providing protective silica hydrogel layer on the microgel surface(More)
Macroscopic adhesion-aggregation, floc formation, and subsequent transportation of microorganisms in porous media are closely related to the microscopic behavior and properties of individual cells. The classical Tabor's parameter in colloidal science is modified to correlate the macroscopic aggregation and microscopic adhesion properties of microorganisms.(More)
The work of adhesion at the interface of electrospun membrane and rigid substrate is measured by a shaft-loaded blister test (SLBT). Poly(vinylidene fluoride) (PVDF) were electrospun with an average fiber diameter of 333 ± 59 nm. Commercial cardboard with inorganic coating was used to provide a model substrate for adhesion tests. In SLBT, the elastic(More)
A simple model of cell-substrate adhesion, in response to osmosis change, is derived to describe quantitatively the interrelationships between osmotic inflation, contact area and angle, membrane stretching and adhesion strength. As the cell inflates, the contact area shrinks in dimension, until the cell is eventually lifted off the substrate. The(More)
It has been recently demonstrated that acyl chain mismatch of phospholipid bilayer composed of a binary lipid mixture induces component formation on the lateral plane of the bilayer [Biophys. J. 83 (2002) 1820-1883]. In this report, the contact mechanics of unilamellar vesicles composed of binary dimyristoyl-phosphatidylcholine(More)
It has been demonstrated that there is a mechanochemical relationship between collagen and collagenolytic enzymes such that increased tensile mechanical strain reduces the enzymatic cutting rate. This mechanochemical relationship has the potential to permit directed remodelling of tissue-engineered constructs in vitro and to shed light on the generation of(More)
We engineered functional cardiac patches by seeding neonatal rat cardiomyocytes onto carbon nanotube (CNT) incorporated photocrosslinkable gelatin methacrylate (GelMA) hydrogel. The resulting cardiac constructs showed excellent mechanical integrity and advanced electrophysiological functions. Specifically, myocardial tissues cultured on 50 μm thick CNTGelMA(More)