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BACKGROUND Detailed knowledge of the subcellular location of each expressed protein is critical to a full understanding of its function. Fluorescence microscopy, in combination with methods for fluorescent tagging, is the most suitable current method for proteome-wide determination of subcellular location. Previous work has shown that neural network(More)
BACKGROUND On March 30, a novel influenza A subtype H7N9 virus (A/H7N9) was detected in patients with severe respiratory disease in eastern China. Virological factors associated with a poor clinical outcome for this virus remain unclear. We quantified the viral load and analysed antiviral resistance mutations in specimens from patients with A/H7N9. (More)
Gene-specific targeting of the Sin3 corepressor complex by DNA-bound repressors is an important mechanism of gene silencing in eukaryotes. The Sin3 corepressor specifically associates with a diverse group of transcriptional repressors, including members of the Mad family, that play crucial roles in development. The NMR structure of the complex formed by the(More)
Continuous integration (CI) systems automate the compilation, building, and testing of software. Despite CI rising as a big success story in automated software engineering, it has received almost no attention from the research community. For example, how widely is CI used in practice, and what are some costs and benefits associated with CI? Without(More)
Fluorescence microscope images capture information from an entire field of view, which often comprises several cells scattered on the slide. We have previously trained classifiers to accurately predict subcellular location patterns by using numerical features calculated from manually cropped 2D single-cell images. We describe here results on directly(More)
Receptor tyrosine kinases and Notch are crucial for tube formation and branching morphogenesis in many systems, but the specific cellular processes that require signaling are poorly understood. Here we describe sequential roles for Notch and Epidermal growth factor (EGF)-Ras-ERK signaling in the development of epithelial tube cells in the C. elegans(More)
AIMS Our aim was to explore the mechanism underlying the transcriptional regulation of adiponectin and its receptors (AdipoR) in cultured rat cardiac fibroblasts. METHODS AND RESULTS Using western blot and real-time RT-PCR assays, the expression of adiponectin and its receptors was determined. Using Southwestern blot and electrophoretic mobility shift(More)
The central goal of proteomics is to clarify the mechanism by which each protein in a given cell type carries out its function. Automated protein subcellular location determination by fluorescence microscopy can play an important role in fulfilling this goal. The subcellular location of a protein is critical to understanding its function because each(More)
In this paper, we introduce a new approach to fingerprint classification based on both singularities and traced pseudoridge analysis. Since noise exists in most of the fingerprint images including those in the NIST databases which are used by many researchers, it is difficult to get the correct number and position of the singulairities such as core or delta(More)