Kadzuki Nakabayashi

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PURPOSE Detection of sentinel lymph node (SLN) metastasis in breast cancer patients has conventionally been determined by intraoperative histopathologic examination of frozen sections followed by definitive postoperative examination of permanent sections. The purpose of this study is to develop a more efficient method for intraoperative detection of lymph(More)
This study was designed to apply safely the sentinel node navigation surgery (SNNS) to the malignancies, an accurate and prompt intraoperative diagnosis of SN is essential, and micrometastasis has been frequently missed by conventional frozen sections. Recently, a novel molecular-based rapid diagnosis for the lymph node (LN) metastases has been developed(More)
We investigated the clinical utility of our novel loop-mediated isothermal amplification (LAMP) assay developed as a rapid molecular diagnostic method, using carcinoembryonic antigen (CEA)-mRNA as a marker for detecting tumor cells in patients with non-small cell lung cancer (NSCLC). We evaluated the sensitivity of our LAMP technique using a known quantity(More)
A sublobar resection is currently recognized as an option for early small-sized non-small cell lung cancer (NSCLC), and intraoperative rapid and accurate lymph node assessment is required for a complete resection. To solve this issue, we investigated the clinical utility of one-step nucleic acid amplification (OSNA) assay, an automated rapid molecular(More)
BACKGROUND We previously reported that the one-step nucleic acid amplification assay is effective for lymph node metastasis detection in breast cancer patients. This paper describes the identification of CK19 mRNA as an optimal marker and its cut-off value for use in the detection of one-step nucleic acid amplification-based lymph node metastasis in(More)
To improve lymph node (LN) metastasis identification for patients with endometrial cancer (EC), this study assessed the usefulness of molecular biologic techniques using a one-step nucleic acid amplification (OSNA) assay. Using quantitative reverse transcription polymerase chain reaction (qRT-PCR), an optimal mRNA marker was selected, and its expression was(More)
OBJECTIVE The purpose of this study was to examine the utility of the one-step nucleic acid amplification (OSNA) assay using cytokeratin (CK) 19 (KRT19) messenger RNA (mRNA) for the detection of sentinel lymph node (SLN) metastases in cervical cancer patients. METHODS To determine a cutoff value, KRT19 mRNA was assessed by OSNA assay using 239 lymph nodes(More)
BREAST CANCER B01: Application of Cytokeratin 19 to Molecular Diagnosis of Sentinel Lymph Nodes: Phenotypic and Immunophenotypic Characteristics of Breast Cancers with no or Low Cytokeratin 19 Expression H. Asakawa, H. Tsuda, J. Omata, S. Ueda, K. Onozato, M. Tsujimoto, Y. Otomo, K. Nakabayashi, K. Fukatsu, H. Mochizuki National Defense Medical College,(More)
BACKGROUND Carcinoembryonic antigen (CEA) mRNA expression in peritoneal washes from gastric cancer patients has been reported as an indicator for survival or peritoneal recurrence. The whole process of CEA mRNA detection is time- and labor-intensive. We report the potential of One-Step Nucleic acid Amplification (OSNA) as a rapid and simple system for CEA(More)
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