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  • K M Lam
  • Journal of comparative pathology
  • 1996
Specific pathogen-free chickens were inoculated with the GB strain of Newcastle disease virus by the ocular route, and were killed at daily intervals after infection. Peripheral blood mononuclear cells (PBMCs) were harvested for DNA extraction, electron microscopy, and flow cytometry studies to detect the presence of cellular apoptosis and necrosis.(More)
The effects of the infectious bursal disease virus (IBDV) on chicken heterophils and macrophages are reported. Short-term incubation of IBDV with heterophils causes an activation of cellular oxidative burst, a decrease in phagocytosis and cellular migration, and an increase in cellular adherence to the IBDV-infected chicken embryo fibroblasts. Under(More)
Nuclear factor of activated T cells 5 protein (NFAT5) is thought to be important for cellular adaptation to osmotic stress by regulating the transcription of genes responsible for the synthesis or transport of organic osmolytes. It is also thought to play a role in immune function, myogenesis and cancer invasion. To better understand the function of NFAT5,(More)
Mycoplasma gallisepticum (MG) S6 strain was treated with nitrosoguanidine to obtain temperature-sensitive mutants. Of the 101 colonies screened, 4 were found to be temperature sensitive. These mutants and the wild type organisms were serologically and morphologically identical. Three of the 4 mutants failed to produce air sac lesions when inoculated(More)
Chemokines, characterized as pro-inflammatory chemicals made by the immune system, consist of a family of low molecular weight proteins with potent in vitro chemotactic activity causing leukocyte accumulation in vivo. This study determines the effects of organochlorine pesticide exposure on the chemotactic functions of monkey neutrophils and monocytes,(More)
Newly hatched chickens were significantly protected against challenge by the virulent S6 strain of Mycoplasma gallisepticum after vaccination with the TS 100 mutant. Removal of the thymus did not abolish the protective effect of the vaccine, but removal of the bursa of Fabricius did. The results suggest that the resistance induced by vaccine is mediated by(More)
  • K M Lam
  • Journal of comparative pathology
  • 2001
An apathogenic strain of duck enteritis virus was injected into the allantoic cavity of duck embryos at 17 or 18 days of incubation. Four to 6 days later, the embryos showed massive infiltration of eosinophilic granulocytes in the spleen. Peroxidase and immunohistochemical labelling showed that the granulocytes were peroxidase-positive and contained major(More)
Heterophils were labeled with fluorescein isothiocyanate and then placed in a modified Boyden chamber to determine their response to chemoattractants. After incubation, the cells that had migrated into the membrane were examined under an epifluorescent microscope, and the image was captured by a charge-coupled digital camera and a frame grabber and saved.(More)
Chicken peripheral blood lymphocytes (PBL) were infected with the GB strain of Newcastle disease virus (NDV). At 0, 1.5 and 3 h postinfection, PBL were stained with acridine orange-ethidium bromide and examined for apoptotic and necrotic indices under a UV microscope. At the end of 3 h, the cells were pelleted and were either fixed for electron microscopy(More)
Fifteen-day-old fertile eggs (specific pathogen-free) were inoculated with the infectious bursal disease virus (IBDV) by the allantoic route and were opened and examined 2, 4 or 6 days later. The bursas of Fabricius (BFs) were collected and processed for DNA extraction, flow cytometry, and light and electron microscopy. Cellular DNA was subjected to(More)