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A midgut trypanolysin and an agglutinin from Glossina palpalis subspecies were isolated and partially characterized using anion-exchange chromatography and polyacrylamide gel electrophoresis. FPLC fractions of midgut extracts of Glossina palpalis palpalis caused agglutination and lysis of two trypanosome species (Trypanosoma congolense and Trypanosoma(More)
Procyclic trypomastigotes of Trypanosoma brucei brucei, cultured in Cunningham's medium with 20% heat-inactivated foetal calf serum at 27 degrees C, attached to chitosan and to gels of N-acetyl chitosan and glycol chitosan. Following attachment, epimastigotes, metacyclic-like trypomastigotes and multinucleate parasites appeared in the culture supernatant.
Phlebotomus ariasi Tonnoir sandflies were caught in light traps hung in oak trees and in a house in the Cévennes focus of leishmaniasis in the South of France. The flies were cryopreserved either immediately on removal from the traps, or after starvation for 6-7 days, or after 6-7 days starvation followed by exposure to oak infested with the aphid genera(More)
Three flagellates recently isolated from the hindguts of tsetse flies in West Africa were compared with a previously described T. grayi-like trypanosome isolated in East Africa. In media with Microtus agrestis feeder layer cells, the flagellates developed into bloodstream-like trypomastigotes, which resembled the description of T. grayi from the blood of(More)
The ultrastructure of the peritrophic membrane of the female sandfly Phlebotomus papatasi has been studied at various times after blood meals. The membrane begins to form within four hours of the blood meal with the secretion by the entire midgut epithelium of an electron-dense amorphous material. Subsequently, the membrane is stabilized and strengthened by(More)
Microtus agrestis embryo fibroblasts (MAEF) support the survival and multiplication at 37 C of the mammalian multiplicative forms of the Herpetosoma trypanosomes Trypanosoma microti, T. evotomys, T. musculi, and T. lewisi passaged from cultures on Schneider's Drosophila medium and of T. grosi from Grace's medium. MAEF layers with parasites were maintained(More)
The first description of an electron microscopic study ofTrypanosoma corvi in the vectorOrnithomyia avicularia is reported. There is a close association between vector and parasite in the midgut, ileum and rectum of the vector. The midgut distribution of parasites is determined by the peritrophic membrane, which confines the early infection to the(More)
Fluorescein-lectin conjugates were used as markers to determine the presence of surface carbohydrates on the peritrophic membranes of 6 Glossina species. Inter- and intra-specific variation in exposed carbohydrate residues was observed. Peritrophic membranes from non-teneral flies appeared to have less exposed surface carbohydrates than those of tenerals.(More)
Lysates of heads, hind- and midguts of male and female Phlebotomus papatasi were found to contain lectins or lectin-like molecules capable of agglutinating human red blood cell types of the ABO(H) system and promastigotes of Leishmania aethiopica, L. major and L. donovani but not L. hertigi hertigi promastigotes or Crithidia fasciculata choanomastigotes.(More)
Trypanosomes were isolated by culture from 2 out of 50 blood samples collected from reindeer (Rangifer tarandus L.) in northern Sweden and from a blood sample from a moose (Alces alces) from Southern Sweden. The parasites were indistinguishable morphologically from other trypanosomes reported from cervids, both as epimastigotes in axenic culture at 27(More)