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During cell wall biogenesis in Saccharomyces cerevisiae, some glycosylphosphatidylinositol (GPI)-attached proteins are detached from GPI moieties and bound to beta-1,6-glucan of the cell wall. The amino acid sequence requirement for the incorporation of GPI-attached proteins into the cell wall was studied by using reporter fusion proteins. Only the short(More)
The final destination of glycosylphosphatidylinositol (GPI)-attached proteins in Saccharomyces cerevisiae is the plasma membrane or the cell wall. Two kinds of signals have been proposed for their cellular localization: (i) the specific amino acid residues V, I, or L at the site 4 or 5 amino acids upstream of the GPI attachment site (the omega site) and Y(More)
The induction of pleomorphism of Lactobacillus bifidus by NaCl was completely inhibited by CaCl(2). When the organism was cultivated in calcium-free medium, growth of the bifid form was exclusively observed. Supplementation of calcium ion in the medium caused bacilloid growth. Chemical analyses indicated that calcium content of the bifid form organisms was(More)
We have investigated how point mutations in the two ATP-binding motifs (G(463)PNGCGK(469)ST and G(701)PNGAGK(707)ST) of elongation factor 3 (EF-3) affect ribosome-activated ATPase activity of EF-3, polyphenylalanine synthesis, and growth of Saccharomyces cerevisiae. The point mutation impaired the ribosome-activated ATPase activity of EF-3, when glycine(463(More)
It is welt known that Lalctobcicillus bifidus often shows branched forms (R. F. Norris, T. Flanders, R. M. Tomarelli, and P. Gyorgy, J. Bacteriol. 60:681, 1950; V. Sundman, K. Bjrokesten, and H. G. Gyllenberpg, J. Gen. Microbiol. 21:371, 1959), but the mnechanism underlying this phenomenon has not yet been clarified. Although it has been reported that the(More)
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