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Live imaging of mitochondrial function is crucial to understand the important role played by these organelles in a wide range of diseases. The mitochondrial redox potential is a particularly informative measure of mitochondrial function, and can be monitored using the endogenous green fluorescence of oxidized mitochondrial flavoproteins. Here, we have(More)
Methods Mice (C57/bl6) were injected intraperitoneally with lipopolysaccharide (LPS) (5 mg/kg; n = 15) or saline (0.01 ml/g; n = 6). Six hours later, the cerebral cortex was exposed and mitochondrial function assessed using the inherent fluorescence of flavin adenine dinucleotide (FAD). The mice spontaneously breathed a sequence of 21% oxygen (room air),(More)
Methods A flap of skull was removed and cerebral cortex exposed in isoflurane-anaesthetised rats undergoing HS (target mean arterial pressure (MAP) of 40 mmHg for 30 minutes) (n = 7) compared with sham (n = 6) controls. Mitochondrial function was assessed by observing mitochondrial membrane potential (using tetramethyl rhodamine methyl ester (TMRM); ex: 543(More)
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