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Eight-week-old mice of 3 sublines of strain C57BL/6 were given s.c. injections of 1,2-dimethylhydrazine (DMH), once weekly for 10 weeks. The highest incidence (85%) of colorectal tumors occurred in C57BL/6N mice. Colorectal tumors occurred in 43% of C57BL/6J mice, while only 3 (10%) C57BL/6Ha mice developed these tumors. Possible factors responsible for the(More)
Protein leakage was used as a quantitative measure of poliovirus-induced cellular injury under suspended cell culture conditions. The requirements for protein leakage were studied in detail and it was established that events early in the infectious cycle which depend upon viral protein synthesis were responsible for cell damage. Extralysosomal(More)
In this brief review, we have illustrated the historical development of the growth media commonly employed for the propagation of cultured mammalian cells. While substantial progress has been achieved, the field may best be described as conservative and pragmatic. To date, the function of many components of the growth medium essential for cellular(More)
Morphologically defined stages of 1,2-dimethylhydrazine (DMH)-induced colorectal carcinogenesis were correlated with concomitant changes in the immunological competence of susceptible SWR/J mice. The morphogenesis of colorectal tumors and the cellular changes occurring in the spleen, drain ing (mesenteric) lymph node, thymus, liver, and lung of mice were(More)
The concept of vaccinating a tumor-bearing individual with a portion of his own tumor, rendered nonproliferative by X-irradiation or other means, dates back to the 1st decade of this century (13). Table 1 categorizes the various methods that have been used to increase the immunogenicity of tumor material. As a general rule, cell-free homogenates of tumors(More)
Inbred mice of strains C57BL/6J (B6) (Ahb/-Ahb) and DBA/2J (D2) (Ahd/Ahd) were administered 3-methylcholanthrene (MCA) alone, 1,2-dimethylhydrazine (DMH) alone, and MCA and DMH together. Colorectal tumors were observed in 32% of the DMH-treated B6 mice, but no such tumors occurred in similarly treated D2 mice. Of the MCA-treated D2 mice, 46% were(More)