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Primordial germ cells (PGCS) are the progenitor cells for the gametes. They can be obtained from avian embryos by dissociating the germinal crescent region, where they accumulate, or by sampling the blood at the time of their migration to the gonad. PGCS were obtained from these sources and transfected with defective retroviruses. These manipulated cells(More)
Certain cells in the hepatopancreas of the common garden snail (Helix aspersa) contain intracellular granules that are sites of metal-ion accumulation. These granules have been extracted and investigated by u.v. and i.r. spectroscopy, atomic-absorption spectroscopy, X-ray microanalysis, thermogravimetric analysis, enzymic assay and microanalysis. The(More)
1. Experiments were undertaken to attempt to sterilise fowl embryos with ultraviolet light. Such sterilised embryos would be useful as recipients of genetically manipulated germ cells. 2. The germinal crescents of embryos were exposed to a calibrated UV source at stages 4 and 8 to 10 of incubation for 30 s, 3 min and 10 min. Teratological and sterility(More)
SUMMARY Eggs of the domestic fowl were injected with 4B Ca and then subjected to normal incubation or to incubation in 9% carbon dioxide in air. The embryos , yolk, albumen and shell were analysed at various times after incubation had started. The results demonstrate that there is normally a resorption of the eggshell during the latter part of incubation(More)
1. A simple artificial diet was devised for larvae of Lucilia sericata Meigen. 2. A basic diet of 20 g/l agar with 20% horse blood and added yeast sustained normal growth and development, as compared with a lamb meat control. 3. When yeast was added to the blood agar diet at 50 g/l, both peak and final larval weights were increased by 25-50% at higher(More)