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Quantification of cell nuclei isolated from hepatocytes by cell lysis with nonionic detergent in citric acid.
A method was developed for determining the number of nuclei of hepatocytes cultured on collagen gel using a nonionic detergent, Nikkol BO-10TX. The cells were recovered in a test tube afterExpand
Suppressor function of patients with systemic lupus erythematosus
Peripheral mononuclear cells from patients with systemic lupus erythematosus (SLE), when incubated for 48 hr with or without concanavalin A (ConA), were found to be capable of inhibiting the pokeweedExpand
Cytotoxic Activity of Lymphocytes: VII. Cellular Origin of α-Lymphotoxin
To detect the cellular origins of α-lymphotoxin (α-LT), we cultured various subpopulations of human blood lymphocytes separated by erythrocyte-rosetting techniques with various mitogens. TExpand
Reevaluation of suppressor cell function in systemic lupus erythematosus.
Abstract This study was undertaken to reevaluate suppressor cell function in patients with systemic lupus erythematosus (SLE). First, we found that preculture of normal peripheral blood mononuclearExpand
Characterization of the spontaneous DNA-synthesizing and/or IgG-secreting cells in peripheral blood from patients with systemic lupus erythematosus.
Characterization of the cells responsible for spontaneous DNA synthesis and/or IgG secretion in systemic lupus erythematosus (SLE) was undertaken by fractionation of the peripheral blood mononuclearExpand
Perfusion cells for studying regional variation in oral mucosal permeability in humans. 2. A specialized transport mechanism in D-glucose absorption exists in dorsum of tongue.
To clarify the site of d-glucose absorption in human oral cavity, newly designed perfusion cells were applied to five different sites in the human oral cavity, i.e., the dorsum of the tongue, theExpand
A double antibody radioimmunoassay for measurement of IgG, IgA and IgM synthesized by human lymphocytes in vitro.
To investigate cellular interactions between human T and B lymphocytes in various diseases, we established a technique to prove terminal differentiation of B lymphocytes into immunoglobulinExpand
Multiple forms of growth inhibitors secreted from cultured rat liver cells: purification and characterization.
It was found that a non-tumorigenic epithelial cell line from the liver of a Buffalo-strain rat (BRL) secreted into the culture medium various inhibitors of the growth of BRL and RSV-BRL (tumorigenicExpand
Growth inhibitors in serum, platelets, and normal and malignant tissues.
The non-malignant epithelial cell line from Buffalo rat liver (BRL) and its malignant transformant obtained by Rous sarcoma virus infection (RSV-BRL) were used as the indicators. A survey was made inExpand
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