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Amplification of the entire kanamycin biosynthetic gene cluster during empirical strain improvement of Streptomyces kanamyceticus.
Streptomyces kanamyceticus 12-6 is a derivative of the wild-type strain developed for industrial kanamycin (Km) production. Southern analysis and DNA sequencing revealed amplification of a largeExpand
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A system for the targeted amplification of bacterial gene clusters multiplies antibiotic yield in Streptomyces coelicolor
Gene clusters found in bacterial species classified as Streptomyces encode the majority of known antibiotics as well as many pharmaceutically active compounds. A site-specific recombination systemExpand
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Molecular Cloning and Characterization of the Fructooligosaccharide-Producing β-Fructofuranosidase Gene from Aspergillus niger ATCC 20611
The fopA gene encoding a fructooligosaccharide-producing β-fructofuranosidase was isolated from Aspergillus niger ATCC 20611. The primary structure deduced from the nucleotide sequence showedExpand
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Cloning and Characterization of Saponin Hydrolases from Aspergillus oryzae and Eupenicillium brefeldianum
We purified saponin hydrolases from Aspergillus oryzae PF1224 and Eupenicillium brefeldianum PF1226. It was confirmed that the enzymes from A. oryzae PF1224 (Sda1) and E. brefeldianum PF1226 (Sde1)Expand
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Preparation of protoplasts of Rhizopus niveus and their transformation with plasmid DNA
An efficient method for protoplast formation of Rhizopus niveus, a filamentous fungus with high capability to secrete enzymes out of the cells, was established using three lytic enzymes inExpand
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A Novel Saponin Hydrolase from Neocosmospora vasinfecta var. vasinfecta
ABSTRACT We isolated a soybean saponin hydrolase from Neocosmospora vasinfecta var. vasinfecta PF1225, a filamentous fungus that can degrade soybean saponin and generate soyasapogenol B. This enzymeExpand
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The integrative transformation of Pleurotus ostreatus using bialaphos resistance as a dominant selectable marker.
A plasmid pLC-bar containing the bialaphos resistance gene derived from Streptomyces hygroscopicus between the Lentinus edodes ras gene promoter and priA gene terminator was constructed. WhenExpand
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Para-position derivatives of fungal anthelmintic cyclodepsipeptides engineered with Streptomyces venezuelae antibiotic biosynthetic genes
PF1022A, a cyclooctadepsipeptide possessing strong anthelmintic properties and produced by the filamentous fungus Rosellinia sp. PF1022, consists of four alternating residues of N-methyl-L-leucineExpand
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The kanamycin biosynthetic gene cluster from Streptomyces kanamyceticus.
Kanamycin is an aminoglycoside antibiotic produced by Streptomyces kanamyceticus1). Although it possesses broad range antibacterial activity, bacteria resistant to this antibiotic readily appear, andExpand
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ZouA, a putative relaxase, is essential for dna amplification in Streptomyces kanamyceticus.
Previously, we showed that a 145-kb DNA region, including the entire kanamycin biosynthetic gene cluster (with two kanamycin resistance genes), was tandemly amplified up to 36-fold in an industrialExpand
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