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Intravaginal gene silencing using biodegradable polymer nanoparticles densely loaded with small-interfering RNA
This is the first report demonstrating that biodegradable polymer nanoparticles are effective delivery vehicles for siRNA in the vaginal mucosa, using nanoparticles composed entirely of FDA-approved materials. Expand
Mucosal vaccine design and delivery.
Discovery of safe and effective mucosal adjuvants are also being sought to enhance the magnitude and quality of the protective immune response. Expand
Current strategies for sustaining drug release from electrospun nanofibers.
This work investigates several existing strategies to sustain release of hydrophilic small molecule drugs that are highly-loaded in electrospun fibers and investigates what is known about the design constraints required to realize multi-day release from fibers fabricated from uniaxial and coaxial electrospinning. Expand
Drug-Eluting Fibers for HIV-1 Inhibition and Contraception
It is shown that drug-eluting fiber meshes designed for topical drug delivery can function as a combination chemical and physical barrier MPT, and a previously unknown activity of glycerol monolaurate to potently inhibit sperm motility and viability is reported. Expand
Increasing PCR Fragment Stability and Protein Yields in a Cell-Free System with Genetically Modified Escherichia coli Extracts
It is shown that cell-free protein synthesis reactions using PCR products amplified from genomic DNA and extended with the T7 promoter and the T8 terminator give the same high yields of proteins in 96-well plates. Expand
Manufacturing scale-up of electrospun poly(vinyl alcohol) fibers containing tenofovir for vaginal drug delivery.
Important solution and processing parameters for scale-up production of TFV drug-eluting fibers by wire electrospinning are defined, which may have significant implications for pharmaceutical manufacturing of fiber-based medical fabrics for clinical use. Expand
Relationships between mechanical properties and drug release from electrospun fibers of PCL and PLGA blends
  • S. Chou, K. Woodrow
  • Materials Science, Medicine
  • Journal of the mechanical behavior of biomedical…
  • 1 January 2017
Mechanical properties of electrospun polycaprolactone and poly (D,L-lactic-co-glycolic) acid (PLGA) fibers at various blend ratios in the presence and absence of a small molecule hydrophilic drug, tenofovir (TFV), provide scientific understanding on the dependence of drug release and drug loading on the mechanical properties of drug-eluting fibers. Expand
Tunable Release of Multiclass Anti-HIV Drugs that are Water-Soluble and Loaded at High Drug Content in Polyester Blended Electrospun Fibers
Tunable release of several water-soluble anti-HIV drugs from electrospun fibers fabricated with blends of two biodegradable polyesters is investigated and it is expected that this system represents an effective strategy to sustain delivery of water- soluble molecules that will benefit a variety of biomedical applications. Expand
Nanoparticle-Based ARV Drug Combinations for Synergistic Inhibition of Cell-Free and Cell-Cell HIV Transmission.
This work shows that physicochemically diverse ARV drugs can be encapsulated within polymeric nanoparticles to deliver multidrug combinations that provide potent HIV chemoprophylaxis in relevant models of cell-free, cell-cell, and mucosal tissue infection and demonstrates that ARV-NP combinations control HIV-1 transmission more efficiently than free-drug combinations. Expand
Rapid expression of functional genomic libraries.
Improvements in the parallel synthesis of linear ETs combined with enhanced in vitro enzyme activation help to make CFPS systems more attractive platforms for high-throughput evaluation of protein function. Expand