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High- and low-mobility populations of HP1 in heterochromatin of mammalian cells.
Heterochromatin protein 1 (HP1) is a conserved nonhistone chromosomal protein with functions in euchromatin and heterochromatin. Here we investigated the diffusional behaviors of HP1 isoforms inExpand
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Composition and Hierarchical Organisation of a Spider Silk
Albeit silks are fairly well understood on a molecular level, their hierarchical organisation and the full complexity of constituents in the spun fibre remain poorly defined. Here we linkExpand
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Characterization of the protein components of Nephila clavipes dragline silk.
Spider silk is predominantly composed of structural proteins called spider fibroins or spidroins. The major ampullate silk that forms the dragline and the cobweb's frame threads of Nephila clavipesExpand
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Abundance and distribution of RNA polymerase II in Arabidopsis interphase nuclei
Highlight Super-resolution microscopy reveals the number of RNA polymerase II molecules in plant interphase nuclei. Both active and inactive polymerase variants aggregate in a range known fromExpand
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The Replication Protein A Binding Site in Simian Virus 40 (SV40) T Antigen and Its Role in the Initial Steps of SV40 DNA Replication
ABSTRACT Physical interactions of simian virus 40 (SV40) large tumor (T) antigen with cellular DNA polymerase α-primase (Pol/Prim) and replication protein A (RPA) appear to be responsible forExpand
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The LSM 510 META - ConfoCor 2 system: an integrated imaging and spectroscopic platform for single-molecule detection.
Fluorescence Correlation Spectroscopy (FCS) has developed into a routine method to quantitatively study diffusion of molecules and kinetic processes. FCS has recently become popular to complementExpand
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Two Regions of Simian Virus 40 T Antigen Determine Cooperativity of Double-Hexamer Assembly on the Viral Origin of DNA Replication and Promote Hexamer Interactions during Bidirectional Origin DNA
ABSTRACT Phosphorylation of simian virus 40 large tumor (T) antigen on threonine 124 is essential for viral DNA replication. A mutant T antigen (T124A), in which this threonine was replaced byExpand
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Initiation of eukaryotic DNA replication: regulation and mechanisms.
The accurate and timely duplication of the genome is a major task for eukaryotic cells. This process requires the cooperation of multiple factors to ensure the stability of the genetic information ofExpand
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Protein-Protein Interactions of the Primase Subunits p58 and p48 with Simian Virus 40 T Antigen Are Required for Efficient Primer Synthesis in a Cell-free System*
DNA polymerase α-primase (pol-prim, consisting of p180-p68-p58-p48), and primase p58-p48 (prim2) synthesize short RNA primers on single-stranded DNA. In the SV40 DNA replication system, only pol-primExpand
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Differential polymerization of the two main protein components of dragline silk during fibre spinning
Spider silks are some of the strongest materials found in nature1,2. Achieving the high tensile strength and elasticity of the dragline of orb-weaving spiders, such as Nephila clavipes3,4,5, is aExpand
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