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Composition and Hierarchical Organisation of a Spider Silk

TLDR
The morphological defined structural elements in dragline silk of Nephila clavipes are linked to their biochemical composition and physicochemical properties to understand the composite nature of silk and its supra-molecular organisation.
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High- and low-mobility populations of HP1 in heterochromatin of mammalian cells.

TLDR
Using fluorescence correlation spectroscopy and fluorescence recovery after photobleaching, data suggest that mobile as well as very slow populations of HP1 may function in concert to maintain a stable conformation of constitutive heterochromatin throughout the cell cycle.
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Characterization of the protein components of Nephila clavipes dragline silk.

TLDR
Spider silk is predominantly composed of structural proteins called spider fibroins or spidroins, and polypeptides reactive against both Masp 1 and 2 specific antibodies were found to be expressed in the epithelia of the tail and different gland zones and accumulated in the gland secreted material.
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The Replication Protein A Binding Site in Simian Virus 40 (SV40) T Antigen and Its Role in the Initial Steps of SV40 DNA Replication

TLDR
The results show that the antibodies specifically inhibited both primer synthesis and primer elongation, demonstrating that this RPA binding site in T antigen plays an essential role in both events.
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The LSM 510 META - ConfoCor 2 system: an integrated imaging and spectroscopic platform for single-molecule detection.

TLDR
An overview of the Zeiss solution of such a combined instrument, the LSM 510 META / ConfoCor 2 system, focuses on the instrumental set up as well as technical advances and improvements in the software that controls FCS data acquisition and evaluation.
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Two Regions of Simian Virus 40 T Antigen Determine Cooperativity of Double-Hexamer Assembly on the Viral Origin of DNA Replication and Promote Hexamer Interactions during Bidirectional Origin DNA…

TLDR
It is proposed that interactions between hexamers play a role in double-hexamer assembly and origin DNA unwinding, and one surface of the DNA binding domain in each subunit of one hexamer may form a docking site that can interact with each sub unit in the other hexamer.
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Differential polymerization of the two main protein components of dragline silk during fibre spinning

TLDR
The findings suggest that the role of spidroin 2 in the spinning process could be to facilitate the formation of fibrils and contribute directly to the elasticity of the silk.
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The conserved C-termini contribute to the properties of spider silk fibroins.

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Correct intranuclear localization of herpes simplex virus DNA polymerase requires the viral ICP8 DNA-binding protein

TLDR
It is demonstrated that a specific nuclear protein can influence the intranuclear location of another nuclear protein.
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Environmental conditions impinge on dragline silk protein composition

TLDR
Analysis of proteolytic peptides and reactivity to spidroin type specific antibodies indicated that MaSp2 constituted only a minor part in the spinning dope as well as in the spun filaments, and the spider might benefit twice, by synthesizing ‘cheaper’ silk that into the bargain has properties that potentially can better support foraging in times of food shortage.
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