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Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome
The in vivo, genetically programmed incorporation of designer amino acids allows the properties of proteins to be tailored with molecular precision. The Methanococcus jannaschii tyrosyl-transfer-RNA… Expand
Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog.
- Daniel T. Rogerson, A. Sachdeva, +8 authors J. Chin
- Biology, Medicine
- Nature chemical biology
- 1 July 2015
Serine phosphorylation is a key post-translational modification that regulates diverse biological processes. Powerful analytical methods have identified thousands of phosphorylation sites, but many… Expand
Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET
The ability to introduce different biophysical probes into defined positions in target proteins will provide powerful approaches for interrogating protein structure, function and dynamics. However,… Expand
Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion
In vivo incorporation of unnatural amino acids by amber codon suppression is limited by release factor-1–mediated peptide chain termination. Orthogonal ribosome-mRNA pairs function in parallel with,… Expand
Robust manufacturing and comprehensive characterization of recombinant hepatitis E virus-like particles in Hecolin(®).
The hepatitis E virus (HEV) vaccine, Hecolin(®), was licensed in China for the prevention of HEV infection and HEV-related diseases with demonstrated safety and efficacy [1,2]. The vaccine is… Expand
Total synthesis of Escherichia coli with a recoded genome
Nature uses 64 codons to encode the synthesis of proteins from the genome, and chooses 1 sense codon—out of up to 6 synonyms—to encode each amino acid. Synonymous codon choice has diverse and… Expand
Concerted, Rapid, Quantitative, and Site-Specific Dual Labeling of Proteins
- A. Sachdeva, K. Wang, T. Elliott, J. Chin
- Medicine, Chemistry
- Journal of the American Chemical Society
- 23 May 2014
Rapid, one-pot, concerted, site-specific labeling of proteins at genetically encoded unnatural amino acids with distinct small molecules at physiological pH, temperature, and pressure is an important… Expand
Defining synonymous codon compression schemes by genome recoding
- K. Wang, Julius Fredens, Simon F Brunner, S. H. Kim, Tiongsun Chia, J. Chin
- Medicine, Biology
- 3 November 2016
Synthetic recoding of genomes, to remove targeted sense codons, may facilitate the encoded cellular synthesis of unnatural polymers by orthogonal translation systems. However, our limited… Expand
Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody
Hepatitis E virus (HEV), a non-enveloped, positive-sense, single-stranded RNA virus, is a major cause of enteric hepatitis. Classified into the family Hepeviridae, HEV comprises four genotypes… Expand
Bacteria expressed hepatitis E virus capsid proteins maintain virion-like epitopes.
The protein encoded by ORF2 in hepatitis E virus (HEV) is the only capsid protein for this single-stranded RNA virus. It was previously shown that 148 aa (aa 459-606) was needed for dimer formation,… Expand